Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4229574..4230193 Replicon chromosome
Accession NZ_CP074541
Organism Klebsiella pneumoniae strain S24_CRE24

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KHV99_RS21110 Protein ID WP_002892050.1
Coordinates 4229975..4230193 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KHV99_RS21105 Protein ID WP_002892066.1
Coordinates 4229574..4229948 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV99_RS21095 (KHV99_21040) 4224726..4225919 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KHV99_RS21100 (KHV99_21045) 4225942..4229088 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KHV99_RS21105 (KHV99_21050) 4229574..4229948 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KHV99_RS21110 (KHV99_21055) 4229975..4230193 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KHV99_RS21115 (KHV99_21060) 4230352..4230918 + 567 WP_002892030.1 maltose O-acetyltransferase -
KHV99_RS21120 (KHV99_21065) 4230890..4231030 - 141 WP_004147370.1 hypothetical protein -
KHV99_RS21125 (KHV99_21070) 4231051..4231521 + 471 WP_002892026.1 YlaC family protein -
KHV99_RS21130 (KHV99_21075) 4231496..4232947 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KHV99_RS21135 (KHV99_21080) 4233048..4233746 + 699 WP_002892021.1 GNAT family protein -
KHV99_RS21140 (KHV99_21085) 4233743..4233883 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KHV99_RS21145 (KHV99_21090) 4233883..4234146 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T202331 WP_002892050.1 NZ_CP074541:4229975-4230193 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T202331 NZ_CP099375:c4341069-4340728 [Citrobacter braakii]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGCGACGCTATGTCTGTCGCCCGTAGCAGTGTGGCAAATGCTGCTTGC
ATGCCTGCTGGAACAACATTATGGCCTGACACTTAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTGCTGGTGGAAAAGTACGAACTGGTTCGTATTGATCGTAGGGGATTC
AACTGGCAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTGCGGGCGCGGCAGGCAACTGGTTTATTGAAAATAAA
TCGTATTAGCGCTGCACAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT202331 WP_002892066.1 NZ_CP074541:4229574-4229948 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT202331 NZ_CP099375:c4341431-4341090 [Citrobacter braakii]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGGCTGAATCGTAATGTGACGCCTTTCTTTGGCGCTCG
TCTGATACAGGAGGGAAACCGACTGCACTATCTGGCTGACCGGGCCAGCATTAGCGGTGAATTCAGTAACGCAGAATCTT
TGAAGTTGGATGAAGTATTCCCACATTTTATCAGCCAGATGGAGTCAATGTTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACGGTGTCACCCTGTACCACAATGGTTTCACTTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCATCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References