Detailed information of TA system TA202317

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5811..6336
• Replicon: plasmid pCRE20_1
• Accession: NZ_CP074540
• Organism: Klebsiella pneumoniae strain S20_CRE20

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: W8UQV6
• Locus tag: KHV98_RS26960
• Protein ID: WP_001568026.1
• Coordinates: 5811..6116 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: KHV98_RS26965
• Protein ID: WP_001568025.1
• Coordinates: 6118..6336 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHV98_RS26930 (KHV98_26855)	1527..2153	+	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-
KHV98_RS26935	2150..2452	+	303	WP_004197636.1	hypothetical protein	-
KHV98_RS26940 (KHV98_26865)	2892..3686	-	795	WP_004197635.1	site-specific integrase	-
KHV98_RS26945 (KHV98_26870)	3884..4900	-	1017	WP_020315256.1	hypothetical protein	-
KHV98_RS26950 (KHV98_26875)	4897..5220	-	324	WP_022644730.1	hypothetical protein	-
KHV98_RS26955 (KHV98_26880)	5247..5642	-	396	WP_017899885.1	hypothetical protein	-
KHV98_RS26960 (KHV98_26885)	5811..6116	-	306	WP_001568026.1	type II toxin-antitoxin system toxin CcdB	Toxin
KHV98_RS26965 (KHV98_26890)	6118..6336	-	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
KHV98_RS26970 (KHV98_26895)	6889..7578	+	690	WP_004193995.1	hypothetical protein	-
KHV98_RS26975 (KHV98_26900)	7610..8299	-	690	WP_020315560.1	RES family NAD+ phosphorylase	-
KHV98_RS26980 (KHV98_26905)	8736..9005	+	270	WP_020804424.1	hypothetical protein	-
KHV98_RS26985 (KHV98_26910)	8993..9568	+	576	WP_020317495.1	hypothetical protein	-
KHV98_RS26990 (KHV98_26915)	9599..10093	+	495	WP_020802777.1	hypothetical protein	-
KHV98_RS26995 (KHV98_26920)	10137..10505	+	369	WP_004197807.1	hypothetical protein	-
KHV98_RS27000 (KHV98_26925)	10539..10742	+	204	WP_004197808.1	HHA domain-containing protein	-
KHV98_RS27005 (KHV98_26930)	10756..10959	+	204	WP_004197809.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-3	-	1..157575	157575

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11645.32 Da
• Isoelectric Point: 5.6919

>T202317 WP_001568026.1 NZ_CP074540:c6116-5811 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T202317 NZ_CP099374:c3012971-3012639 [Citrobacter braakii]
ATGGACAGAGGGGAAATCTGGCTCGTCTCACTGGATCCTACAGCCGGACATGAACAAAGCGGAAAACGTCCGGTACTTAT
CGTTTCTCCGGCCTCATTTAATAAGTTCACCCGGCTACCCGTTGTCGTTCCCGTCACCAGCGGCGGAAACTTTGCCCGCG
CAGCCGGTTTTACCGTCTCCCTGGACGGTGTGGGAACAAAAACAACGGGCGTTATTCGCTGCGACCAACCCAGAACGATT
GATATGGGGGCCCGGAATGGTAAGCGTCTGGAACGTATACCCGAAGCTGTCGTAAATGAAGTGCTGGCCCGGCTGGAAGC
CATTCTAAGCTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT202317 WP_001568025.1 NZ_CP074540:c6336-6118 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT202317 NZ_CP099374:c3013229-3012972 [Citrobacter braakii]
ATGTATACCACTCGCCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTTCCTCCCGCCGTGTTGAAAACGCTGGCTCT
GTCGACAGACAGCGAAGTGGGAATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAACTACTGGCGCAGTGCGATCCGCACGCCGAAATGAGTGAAGAGGAGCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATCCTGTAA

Structures

Toxin

• AlphaFold DB: A0A514EZN1

Antitoxin

• AlphaFold DB: A0A2J4ZZP8