Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4797947..4798757 Replicon chromosome
Accession NZ_CP074539
Organism Klebsiella pneumoniae strain S20_CRE20

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag KHV98_RS23740 Protein ID WP_002887280.1
Coordinates 4797947..4798480 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KHV98_RS23745 Protein ID WP_002887278.1
Coordinates 4798491..4798757 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV98_RS23735 (KHV98_23670) 4796778..4797899 + 1122 WP_002887282.1 cupin domain-containing protein -
KHV98_RS23740 (KHV98_23675) 4797947..4798480 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
KHV98_RS23745 (KHV98_23680) 4798491..4798757 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KHV98_RS23750 (KHV98_23685) 4798860..4800293 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
KHV98_RS23755 (KHV98_23690) 4800283..4800966 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KHV98_RS23760 (KHV98_23695) 4801138..4802523 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
KHV98_RS23765 (KHV98_23700) 4802541..4802885 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T202313 WP_002887280.1 NZ_CP074539:c4798480-4797947 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T202313 NZ_CP099373:c5070842-5070531 [Citrobacter portucalensis]
ATGCTATTCATAGAAACGGAAATCTTCACTGAAGACGTCAAAAAATTACTGGATGATGATGAATACCGTAGATTACAGAT
ATTCCTTGCGATTCAGCCTGATTGTGGCGATCTGATACAAGATACAGGGGGCCTGAGAAAGGTTCGCTGGAGAGCGCGAG
GGAAAGGAAAACGTAGCGGTGTGAGGATTATCTACTTTCATCAAATCAGAAAATCACAAATTCGATTGCTGCTGATTTAT
CAGAAAGGGATCAAGGATGATCTGACTCCACAAGAAAAAGTGCTGCTGCGTATGTTGAATGAGGGGTGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT202313 WP_002887278.1 NZ_CP074539:c4798757-4798491 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT202313 NZ_CP099373:c5070530-5070243 [Citrobacter portucalensis]
ATGGATAAGGCACTATTTGAGCGATTAACTCAAAGTATGATTCAGATGAATGAGATCGCTGAAGGAACGCGGCAGCCTTC
TCGCACCTTTCATATTGACGCCATGAAAATAAAGGAAATCCGCCAGTCTTCAGGATTATCCCAGACTCGTTTCGCAGATT
TGATATCGGTAAGCGTAGATACGTTGCGAAATTGGGAGCAGGGGCGTCGTTCACCGACCGGTCCAGCAAAAGCGTTGTTA
CGTGCCATTGCCAATGATCCAAAACATGTCATTCAGGCGCTAAGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References