Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4185258..4185877 Replicon chromosome
Accession NZ_CP074539
Organism Klebsiella pneumoniae strain S20_CRE20

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KHV98_RS20795 Protein ID WP_002892050.1
Coordinates 4185659..4185877 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KHV98_RS20790 Protein ID WP_002892066.1
Coordinates 4185258..4185632 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV98_RS20780 (KHV98_20715) 4180410..4181603 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KHV98_RS20785 (KHV98_20720) 4181626..4184772 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KHV98_RS20790 (KHV98_20725) 4185258..4185632 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KHV98_RS20795 (KHV98_20730) 4185659..4185877 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KHV98_RS20800 (KHV98_20735) 4186036..4186602 + 567 WP_002892030.1 maltose O-acetyltransferase -
KHV98_RS20805 (KHV98_20740) 4186574..4186714 - 141 WP_004147370.1 hypothetical protein -
KHV98_RS20810 (KHV98_20745) 4186735..4187205 + 471 WP_002892026.1 YlaC family protein -
KHV98_RS20815 (KHV98_20750) 4187180..4188631 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KHV98_RS20820 (KHV98_20755) 4188732..4189430 + 699 WP_002892021.1 GNAT family protein -
KHV98_RS20825 (KHV98_20760) 4189427..4189567 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KHV98_RS20830 (KHV98_20765) 4189567..4189830 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T202312 WP_002892050.1 NZ_CP074539:4185659-4185877 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T202312 NZ_CP099373:4817978-4818265 [Citrobacter portucalensis]
ATGAGTTATACGGTAAAGTTCAGGGAAGATGCGCTGAAAGAATGGCAAAAACTTGATAAAGCCATTCAGCAGCAATTTGC
CAAAAAACTAAAGAAATGCTGTGAAAATCCACATGTCCCTTCAGCAAAGCTCAGGGGAATAAAAGATTGCTACAAAATCA
AACTACGAACATCAGGGTTTCGTCTTGTTTATCAAGTCATTGACGACACCCTGGTCATCGCGGTCGTTGCCGTCGGCAAA
CGCGAGCGTAGTGAAGTCTATAACCTTGCCAGCGAAAGATTACGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT202312 WP_002892066.1 NZ_CP074539:4185258-4185632 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT202312 NZ_CP099373:4817730-4817981 [Citrobacter portucalensis]
ATGCCGCATATCATTTTAAGCGATACCACCGCCAGTGTGAGTGAGCTGAAAAAGAACCCGATGGCGACGGTTAGCGCAGG
GGATGGGTTTCCGGTCGCAATCCTGAACCGTAACCAACCCGCCTTTTATTGTGTGCCAGCAGAGCTTTACGAAAAAATGC
TCGATGCGCTGGATGACCAACAGCTGGTTAAATTAGTCCATGAACGTAGCCAACAACCCCTGGTGGATGTGGATCTGGAC
AGCTACTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References