Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 46870..47513 Replicon plasmid pCRE16_2
Accession NZ_CP074537
Organism Klebsiella pneumoniae strain S16_CRE16

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag KHV97_RS28945 Protein ID WP_001044770.1
Coordinates 47097..47513 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag KHV97_RS28940 Protein ID WP_001261282.1
Coordinates 46870..47100 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV97_RS28905 (KHV97_28820) 42587..43342 - 756 WP_012539983.1 replication initiation protein RepE -
KHV97_RS28910 (KHV97_28825) 43973..44086 + 114 WP_032072094.1 hypothetical protein -
KHV97_RS28915 (KHV97_28830) 44215..44472 + 258 WP_032408936.1 hypothetical protein -
KHV97_RS28920 (KHV97_28835) 44530..45315 - 786 WP_022644886.1 site-specific integrase -
KHV97_RS28925 (KHV97_28840) 45318..45956 - 639 WP_022644887.1 hypothetical protein -
KHV97_RS28930 (KHV97_28845) 46005..46325 - 321 WP_032072060.1 hypothetical protein -
KHV97_RS28935 (KHV97_28850) 46453..46913 - 461 Protein_52 hypothetical protein -
KHV97_RS28940 (KHV97_28855) 46870..47100 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KHV97_RS28945 (KHV97_28860) 47097..47513 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
KHV97_RS28950 (KHV97_28865) 47587..47805 + 219 Protein_55 AAA family ATPase -
KHV97_RS28955 (KHV97_28870) 47870..48574 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
KHV97_RS28960 (KHV97_28875) 48627..49394 - 768 Protein_57 class 1 integron integrase IntI1 -
KHV97_RS28965 (KHV97_28880) 49550..50023 + 474 WP_004201280.1 trimethoprim-resistant dihydrofolate reductase DfrA14 -
KHV97_RS28970 50244..50360 + 117 Protein_59 plasmid mobilization relaxosome protein MobC -
KHV97_RS28975 50470..50535 + 66 Protein_60 EAL domain-containing protein -
KHV97_RS28980 (KHV97_28890) 50653..51417 + 765 WP_001389365.1 IS6-like element IS6100 family transposase -
KHV97_RS28985 (KHV97_28895) 51557..51622 - 66 Protein_62 EAL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-3 / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib - 1..75610 75610


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T202298 WP_001044770.1 NZ_CP074537:47097-47513 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T202298 NZ_CP099370:2654009-2654491 [Citrobacter portucalensis]
GTGGGATTTGTAACCGCACCTGAGCCTCTAACTCACATTCATCGATTGGCGGAATTTGTCAGCGGAGAAATTATTCTCGA
TGATTGGTTAAAGCAAAAAGGGCTAAAAAATCAGGCTCTCGGCGCAGCCAGGACGTTTGTTGTCTGCAAAACAGACACGC
GACAAGTCGCTGGCTTTTATTCACTGGCCACAGGTAGCGTCAACCACACAGAAGCAACAGGCAATCTTCGGCGTAACATG
CCAGATCCAATCCCCGTAATTATCCTTGCCAGATTGGCCGTAGATCTTTCTGTTCGTGGAAAAGGGTTAGGGGCCGATCT
GCTTCACGATGCGGTACTGCGGTGTTACAAAGTGGCAGAAAATATTGGCGTGCGTGCCGTTATGGTACATGCGCTCACAG
ACGAAGCTAAACGTTTTTATCTTCACCACGGATTCAAAGCATCTCAACTGCAGGAACGCACTCTGTTCCTCAGGCTTTCA
TGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT202298 WP_001261282.1 NZ_CP074537:46870-47100 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT202298 NZ_CP099370:2653755-2654021 [Citrobacter portucalensis]
ATGAAATCAGATGTCCAGCTCAACCTTAGAGCTAAAGAATCCCAACGCGCGCTTATTGATGCGGCCGCAGAGATTCTACA
TAAATCGCGTACAGACTTTATTCTGGAAATGGCCTGCCAGGCGGCAGAGAACGTTATTCTCGATCGCCGAGTGTTTAATT
TTAATGATGAACAATACGCTGAGTTTATCGAGATGCTTGATGCTCCGGTTGCCGAAGATCCCGCTATCAGGAAGCTACTG
GCAAGGAAACCACAGTGGGATTTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References