Detailed information of TA system TA202297

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 53658..54394
• Replicon: plasmid pCRE16_1
• Accession: NZ_CP074536
• Organism: Klebsiella pneumoniae strain S16_CRE16

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: KHV97_RS27830
• Protein ID: WP_003026803.1
• Coordinates: 53912..54394 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: KHV97_RS27825
• Protein ID: WP_003026799.1
• Coordinates: 53658..53924 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHV97_RS27780 (KHV97_27720)	49720..50082	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
KHV97_RS27785 (KHV97_27725)	50132..50482	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
KHV97_RS27790 (KHV97_27730)	50840..51109	+	270	WP_004152102.1	hypothetical protein	-
KHV97_RS27795 (KHV97_27735)	51097..51672	+	576	WP_004152103.1	hypothetical protein	-
KHV97_RS27800 (KHV97_27740)	51703..52197	+	495	WP_004152104.1	DNA-binding protein	-
KHV97_RS27805 (KHV97_27745)	52241..52609	+	369	WP_004152105.1	hypothetical protein	-
KHV97_RS27810 (KHV97_27750)	52643..52846	+	204	WP_004152106.1	HHA domain-containing protein	-
KHV97_RS27815 (KHV97_27755)	52895..53152	+	258	WP_004152107.1	hypothetical protein	-
KHV97_RS27820 (KHV97_27760)	53228..53482	+	255	WP_004152108.1	hypothetical protein	-
KHV97_RS27825 (KHV97_27765)	53658..53924	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
KHV97_RS27830 (KHV97_27770)	53912..54394	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
KHV97_RS27835 (KHV97_27775)	54606..55952	+	1347	WP_020314316.1	ISNCY family transposase	-
KHV97_RS27840	56113..56244	+	132	WP_004218042.1	hypothetical protein	-
KHV97_RS27845 (KHV97_27780)	56453..57628	-	1176	Protein_59	IS3-like element ISKpn38 family transposase	-
KHV97_RS27850 (KHV97_27785)	57805..58767	-	963	WP_004152113.1	zinc metalloprotease HtpX	-
KHV97_RS27855 (KHV97_27790)	58754..59242	-	489	WP_004152114.1	phosphate-starvation-inducible PsiE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / catA1	-	1..212193	212193

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T202297 WP_003026803.1 NZ_CP074536:53912-54394 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T202297 NZ_CP099370:2211807-2211910 [Citrobacter portucalensis]
GGCAGGGTAACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT202297 WP_003026799.1 NZ_CP074536:53658-53924 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT202297 NZ_CP099370:c2211915-2211772 [Citrobacter portucalensis]
CACATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTAAGTTACCCTGCCATTTAAGAATAGATGACAGCGCCAGGTTTTCCAGT

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66