Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5516084..5516709 Replicon chromosome
Accession NZ_CP074535
Organism Klebsiella pneumoniae strain S16_CRE16

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KHV97_RS27185 Protein ID WP_002882817.1
Coordinates 5516084..5516467 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KHV97_RS27190 Protein ID WP_004150355.1
Coordinates 5516467..5516709 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV97_RS27170 (KHV97_27115) 5513450..5514352 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KHV97_RS27175 (KHV97_27120) 5514349..5514984 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KHV97_RS27180 (KHV97_27125) 5514981..5515910 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KHV97_RS27185 (KHV97_27130) 5516084..5516467 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KHV97_RS27190 (KHV97_27135) 5516467..5516709 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KHV97_RS27195 (KHV97_27140) 5516914..5517831 + 918 WP_002882812.1 alpha/beta hydrolase -
KHV97_RS27200 (KHV97_27145) 5517845..5518786 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KHV97_RS27205 (KHV97_27150) 5518831..5519268 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KHV97_RS27210 (KHV97_27155) 5519265..5520125 - 861 WP_002882807.1 virulence factor BrkB family protein -
KHV97_RS27215 (KHV97_27160) 5520119..5520718 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T202296 WP_002882817.1 NZ_CP074535:c5516467-5516084 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T202296 NZ_CP099370:837792-838199 [Citrobacter portucalensis]
GTGGTCCAGTGGCAATCTGATTTACGCGTATCATGGCGCGCGCAGTGGCTTTCACTGCTAATTCATGGGCTGGTCGCGGT
ATTTATTTTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGCTGGTTTTGCTCTCTCTGGTGGTGTTCGATAGCG
TTCGCAGTCAGCGTCGAATCAACGCCTGTCAGGGTGAGATTCGGCTATTGATGGATGGCCGTCTGCGCTGGCAAGGGCAA
GAGTGGTCTATCGTTAGCGCCCCCTGGATGGTTAAAACAGGCATGATGCTGCGTTTGCGATCTGATGCCGGAAAACGTCA
ACATTTGTGGCTGGCAGCAGACAGTATGGATGATGCAGAGTGGCGGGATTTACGGCGGTTGATGCTGCAAAAGGCGAAGC
AAGGGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT202296 WP_004150355.1 NZ_CP074535:c5516709-5516467 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT202296 NZ_CP099370:837545-837811 [Citrobacter portucalensis]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGCCGCGGTATGCGCGAGCTGGACATCTCGATCATGCCGTT
TTTCGAACATGAATATGACAGCTTAAGTGACGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGATGATCCAGATT
TATTTAACTGGTTGATGAATCATGGGAAACCAGCTGATGAGCAGCTGGAACAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References