Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4927505..4928315 Replicon chromosome
Accession NZ_CP074535
Organism Klebsiella pneumoniae strain S16_CRE16

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag KHV97_RS24350 Protein ID WP_002887280.1
Coordinates 4927505..4928038 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KHV97_RS24355 Protein ID WP_002887278.1
Coordinates 4928049..4928315 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV97_RS24345 (KHV97_24300) 4926336..4927457 + 1122 WP_002887282.1 cupin domain-containing protein -
KHV97_RS24350 (KHV97_24305) 4927505..4928038 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
KHV97_RS24355 (KHV97_24310) 4928049..4928315 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KHV97_RS24360 (KHV97_24315) 4928418..4929851 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
KHV97_RS24365 (KHV97_24320) 4929841..4930524 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KHV97_RS24370 (KHV97_24325) 4930696..4932081 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
KHV97_RS24375 (KHV97_24330) 4932099..4932443 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T202293 WP_002887280.1 NZ_CP074535:c4928038-4927505 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T202293 NZ_CP099367:c4697113-4696811 [Citrobacter portucalensis]
ATGTCTAAATCATTAAATATCAGAAATTTTCGGGATACATGGCTTGAAGATTTTTTTGAACGGGCGACATCGCACAGAAA
AATACCTGCGGATATTCACACTGCATTAGCCAGAAAGCTGGATATCATTAATGCGGCTGTTTCGCATCGGGATTTGCGCT
CGCCACCCGGTAACAGATATGAAGAGTTAACCGGAAAATTACAGGAATATTCTTCAATCAGAGTAAACAAGCAGTACCGA
TTAATATTTAAATGGATTAATGGTAAGGCCGAAGATGTGTACTTAGATCCACACATCTATTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT202293 WP_002887278.1 NZ_CP074535:c4928315-4928049 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT202293 NZ_CP099367:c4696790-4696449 [Citrobacter portucalensis]
ATGAAACAGGCATCCAGAAAACCAACGACTGTGGGTGATATCCTGCAATATGAATACCTGGAACCGCTTGATCTGAAAAT
CAACGATCTGGCTGAAATTCTGCATGTTCACCGCAATACAGTGAGCGCACTGGTCAACAATAATCGCAAATTAACAACGG
ATATGGCGTTTCGTTTGGCAAAAGCGTTCGATACCTCCGTTGATTTCTGGCGTAACCTACAGGCGGCGGTTGACCTGTGG
GAAGTTGAAAATGACATGCGGGCGCAAGAAGAACTGAACCGGATTGTTTCAGTTAAAGATTTTATTGCGCAGCGAGATGT
CGGCGCAAAAAAAGTCGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References