Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4314814..4315433 Replicon chromosome
Accession NZ_CP074535
Organism Klebsiella pneumoniae strain S16_CRE16

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KHV97_RS21405 Protein ID WP_002892050.1
Coordinates 4315215..4315433 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KHV97_RS21400 Protein ID WP_002892066.1
Coordinates 4314814..4315188 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV97_RS21390 (KHV97_21345) 4309966..4311159 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KHV97_RS21395 (KHV97_21350) 4311182..4314328 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KHV97_RS21400 (KHV97_21355) 4314814..4315188 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KHV97_RS21405 (KHV97_21360) 4315215..4315433 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KHV97_RS21410 (KHV97_21365) 4315592..4316158 + 567 WP_002892030.1 maltose O-acetyltransferase -
KHV97_RS21415 (KHV97_21370) 4316130..4316270 - 141 WP_004147370.1 hypothetical protein -
KHV97_RS21420 (KHV97_21375) 4316291..4316761 + 471 WP_002892026.1 YlaC family protein -
KHV97_RS21425 (KHV97_21380) 4316736..4318187 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KHV97_RS21430 (KHV97_21385) 4318288..4318986 + 699 WP_002892021.1 GNAT family protein -
KHV97_RS21435 (KHV97_21390) 4318983..4319123 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KHV97_RS21440 (KHV97_21395) 4319123..4319386 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T202292 WP_002892050.1 NZ_CP074535:4315215-4315433 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T202292 NZ_CP099367:3882967-3883185 [Citrobacter portucalensis]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT202292 WP_002892066.1 NZ_CP074535:4314814-4315188 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT202292 NZ_CP099367:3882565-3882939 [Citrobacter portucalensis]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCCACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATTGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References