Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 11580..12223 Replicon plasmid pCRE14_3
Accession NZ_CP074531
Organism Klebsiella pneumoniae strain S14_CRE14

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag KHV96_RS28130 Protein ID WP_001044770.1
Coordinates 11807..12223 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag KHV96_RS28125 Protein ID WP_001261282.1
Coordinates 11580..11810 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV96_RS28090 (KHV96_27965) 7152..8018 - 867 WP_004118283.1 replication initiation protein -
KHV96_RS28095 (KHV96_27970) 8553..8657 + 105 WP_032409716.1 hypothetical protein -
KHV96_RS28100 (KHV96_27975) 8786..9043 + 258 WP_000764642.1 hypothetical protein -
KHV96_RS28105 (KHV96_27980) 9101..9877 - 777 WP_000015958.1 site-specific integrase -
KHV96_RS28110 (KHV96_27985) 9874..10617 - 744 WP_000129823.1 hypothetical protein -
KHV96_RS28115 (KHV96_27990) 10668..11018 - 351 WP_000493378.1 hypothetical protein -
KHV96_RS28120 (KHV96_27995) 11162..11623 - 462 WP_074192227.1 hypothetical protein -
KHV96_RS28125 (KHV96_28000) 11580..11810 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KHV96_RS28130 (KHV96_28005) 11807..12223 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
KHV96_RS28135 (KHV96_28010) 12388..13085 + 698 WP_223174630.1 IS1 family transposase -
KHV96_RS28140 (KHV96_28015) 13233..16250 - 3018 WP_004199413.1 Tn3-like element IS3000 family transposase -
KHV96_RS28145 (KHV96_28020) 16393..16686 + 294 Protein_17 organomercurial transporter MerC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / blaCTX-M-15 / dfrA14 / aph(6)-Id / aph(3'')-Ib / sul2 / blaOXA-1 / aac(6')-Ib-cr - 1..74537 74537


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T202278 WP_001044770.1 NZ_CP074531:11807-12223 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T202278 NZ_CP099365:25489-25770 [Citrobacter braakii]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT202278 WP_001261282.1 NZ_CP074531:11580-11810 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT202278 NZ_CP099365:25248-25499 [Citrobacter braakii]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References