Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 7386..7911 Replicon plasmid pCRE14_2
Accession NZ_CP074530
Organism Klebsiella pneumoniae strain S14_CRE14

Toxin (Protein)


Gene name ccdB Uniprot ID W8EAA6
Locus tag KHV96_RS27460 Protein ID WP_008322213.1
Coordinates 7386..7691 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A6C0NE25
Locus tag KHV96_RS27465 Protein ID WP_032934863.1
Coordinates 7693..7911 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV96_RS27430 (KHV96_27320) 2523..2639 + 117 Protein_2 IS5 family transposase -
KHV96_RS27435 (KHV96_27325) 2711..3831 + 1121 WP_089046468.1 IS3-like element ISSen4 family transposase -
KHV96_RS27440 (KHV96_27330) 3860..4606 + 747 Protein_4 IS5-like element IS5 family transposase -
KHV96_RS27445 (KHV96_27335) 4768..6090 + 1323 WP_016479957.1 GntP family transporter -
KHV96_RS27450 (KHV96_27340) 6289..7080 - 792 WP_008322208.1 site-specific integrase -
KHV96_RS27455 (KHV96_27345) 7070..7384 - 315 WP_008322211.1 hypothetical protein -
KHV96_RS27460 (KHV96_27350) 7386..7691 - 306 WP_008322213.1 type II toxin-antitoxin system toxin CcdB Toxin
KHV96_RS27465 (KHV96_27355) 7693..7911 - 219 WP_032934863.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KHV96_RS27475 (KHV96_27365) 9683..12265 + 2583 WP_016479955.1 EAL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / rmtC / blaNDM-1 htpB 1..110787 110787
- inside IScluster/Tn - - 2523..24556 22033


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11450.23 Da        Isoelectric Point: 5.8211

>T202277 WP_008322213.1 NZ_CP074530:c7691-7386 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLVSARLLSDKVSRELYPVVSVGGDPYRLMTTDMASVPATVIGEEV
ADLSHLENDIQNAINLMFRGI

Download         Length: 306 bp

>T202277 NZ_CP099365:598-909 [Citrobacter braakii]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCAATATCCCAACGATTCGTTAGCCGTTCGGGCGCTGTA
CCGCTTAGTCCGCGAGACGGACTTTTCTTCTCCAGCTGAGATGCGAACGCTGATACCGAGCCTGGACAATTTTAAGTATC
GTAATAAGTGGTGGGTGTTGGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTGAATAAACGCTTC
TATGTGAAGCACATCGCCACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8297.11 Da        Isoelectric Point: 4.4013

>AT202277 WP_032934863.1 NZ_CP074530:c7911-7693 [Klebsiella pneumoniae]
VKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADENRDW

Download         Length: 219 bp

>AT202277 NZ_CP099365:906-1325 [Citrobacter braakii]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCTGTTCCCTTCCTGGGGGGCAGTTCGTC
CGAGAGCGATTACCTCGAAGCGATAGAGTTGGTGGACTATCTGATCGAAAACGACGACGAGAACCCGCTTATTGACTTCC
TGGCGAGTAAAATTGCTGACTATGAGGATAACAGCCCTCGTTTCGCTGAATTCAACAAGGCTATCGCTGAAATGCCAGTC
GGCGTTGCCCTTCTGCGCACCCTGATTGACCAGTACAGGCTGTCTTATTCTGACCTTAAGGAGGAGATCGGCTCTAAATC
GCTGGTTAGCCAGATTCTCTCTGGTCAGCGGTCACTGACAATCATGCACATTAAGGCGCTGTCTGCTCGTTTTGGTGTCA
AACCAGAGTGGTTCCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE27

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE25

References