Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 40954..41714 Replicon plasmid pCRE14_1
Accession NZ_CP074529
Organism Klebsiella pneumoniae strain S14_CRE14

Toxin (Protein)


Gene name tacT Uniprot ID -
Locus tag KHV96_RS26770 Protein ID WP_064177585.1
Coordinates 41208..41714 (+) Length 169 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KHV96_RS26765 Protein ID WP_003026799.1
Coordinates 40954..41220 (+) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV96_RS26720 36440..36602 - 163 Protein_35 type II toxin-antitoxin system RelE/ParE family toxin -
KHV96_RS26725 (KHV96_26635) 36724..36903 - 180 WP_065520248.1 type II toxin-antitoxin system ParD family antitoxin -
KHV96_RS26730 (KHV96_26640) 37216..37461 + 246 WP_064177598.1 hypothetical protein -
KHV96_RS26735 (KHV96_26645) 37466..38038 + 573 WP_064177597.1 hypothetical protein -
KHV96_RS26740 (KHV96_26650) 38069..38563 + 495 WP_064177596.1 hypothetical protein -
KHV96_RS26745 (KHV96_26655) 38624..38827 + 204 WP_004213596.1 HHA domain-containing protein -
KHV96_RS26750 (KHV96_26660) 38841..39071 + 231 WP_049118762.1 hypothetical protein -
KHV96_RS26760 (KHV96_26670) 40524..40778 + 255 WP_044246670.1 hypothetical protein -
KHV96_RS26765 (KHV96_26675) 40954..41220 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KHV96_RS26770 (KHV96_26680) 41208..41714 + 507 WP_064177585.1 GNAT family N-acetyltransferase Toxin
KHV96_RS26775 (KHV96_26685) 41897..43243 + 1347 WP_077255522.1 ISNCY family transposase -
KHV96_RS26780 (KHV96_26690) 43292..43690 + 399 WP_032422684.1 helix-turn-helix domain-containing protein -
KHV96_RS26785 (KHV96_26695) 43870..44901 + 1032 WP_046092923.1 IS630-like element ISEc33 family transposase -
KHV96_RS26790 44993..45103 - 111 Protein_49 IS3 family transposase -
KHV96_RS26795 45119..45268 - 150 Protein_50 transposase -
KHV96_RS26800 (KHV96_26705) 45378..46317 + 940 Protein_51 ISNCY family transposase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..158574 158574
- inside IScluster/Tn - - 33892..46335 12443


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(74-149)

Antitoxin

(6-82)


Sequences


Toxin        


Download         Length: 169 a.a.        Molecular weight: 18119.99 Da        Isoelectric Point: 9.6090

>T202276 WP_064177585.1 NZ_CP074529:41208-41714 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLRQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP
RLIIGVGK

Download         Length: 507 bp

>T202276 NZ_CP099364:c4719799-4719488 [Citrobacter braakii]
ATGCTATTCATAGAAACGGAAATATTCACTGAAGACGTCAAAAAATTACTGGATGATGATGAATACCGTAGATTACAGAT
ATTCCTTGCGATTCAGCCCGATTGTGGCGATCTAATACAAGAAACGGGGGGCCTGAGAAAGGTTCGCTGGAGAGCGCGAG
GGAAAGGAAAACGTGGTGGTGTGAGGATTATCTATTTTCATCAAATCAGGAAATCACAAATTCGATTGTTGCTGATTTAT
CAGAAAGGGATTAAGGATGATCTGACTCCGCAAGAAAAAGTACTGCTGCGTATGTTGAATGAGAGGTGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT202276 WP_003026799.1 NZ_CP074529:40954-41220 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT202276 NZ_CP099364:c4719487-4719200 [Citrobacter braakii]
ATGGATAAGGCACTATTTGAGCGACTAACTCAAAGTATGGTTCAGATGAATGAGATCGCTGAAGGAACACGGCAACCTTC
CCGTACTTTTCATATTGATGCCATGAAAATAAAGGAAATCCGCCTGTCTTCAGGATTATCACAGACTCGTTTCGCAGATT
TGATATCGGTAAGCGTGGATACGTTGCGCAATTGGGAGCAGGGGCGTCGTTCACCGACTGGTCCAGCAAAAGCGTTGTTA
CGTGCCATTGCCAATGATCCAAAACATGTCATTCAGGCGCTAAGATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References