Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 7840..8365 Replicon plasmid pCRE14_1
Accession NZ_CP074529
Organism Klebsiella pneumoniae strain S14_CRE14

Toxin (Protein)


Gene name ccdB Uniprot ID W8EAA6
Locus tag KHV96_RS26585 Protein ID WP_008322213.1
Coordinates 7840..8145 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A6C0NE25
Locus tag KHV96_RS26590 Protein ID WP_032934863.1
Coordinates 8147..8365 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV96_RS26555 (KHV96_26465) 2977..3093 + 117 Protein_2 IS5 family transposase -
KHV96_RS26560 (KHV96_26470) 3165..4285 + 1121 WP_089046468.1 IS3-like element ISSen4 family transposase -
KHV96_RS26565 (KHV96_26475) 4314..5060 + 747 Protein_4 IS5-like element IS5 family transposase -
KHV96_RS26570 (KHV96_26480) 5204..6544 + 1341 Protein_5 GntP family transporter -
KHV96_RS26575 (KHV96_26485) 6743..7534 - 792 WP_008322208.1 site-specific integrase -
KHV96_RS26580 (KHV96_26490) 7524..7838 - 315 WP_008322211.1 hypothetical protein -
KHV96_RS26585 (KHV96_26495) 7840..8145 - 306 WP_008322213.1 type II toxin-antitoxin system toxin CcdB Toxin
KHV96_RS26590 (KHV96_26500) 8147..8365 - 219 WP_032934863.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KHV96_RS26595 (KHV96_26505) 9076..10050 - 975 WP_004098971.1 sensor domain-containing diguanylate cyclase -
KHV96_RS26600 (KHV96_26510) 10687..11595 + 909 WP_004098969.1 HNH endonuclease -
KHV96_RS26605 (KHV96_26515) 11984..12334 - 351 WP_270104539.1 DUF305 domain-containing protein -
KHV96_RS26610 (KHV96_26520) 12478..12909 - 432 WP_000790483.1 silver-binding protein SilE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..158574 158574


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11450.23 Da        Isoelectric Point: 5.8211

>T202275 WP_008322213.1 NZ_CP074529:c8145-7840 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLVSARLLSDKVSRELYPVVSVGGDPYRLMTTDMASVPATVIGEEV
ADLSHLENDIQNAINLMFRGI

Download         Length: 306 bp

>T202275 NZ_CP099364:c4281142-4280861 [Citrobacter braakii]
ATGACCTATAACCTGGAGTTTTTAGATGTTGCGCTCAAGGAGTGGCGCAAGCTTTCCCCTGCATTACGTGAACAGTTCAA
AAAGAAACTGGCGGAGCGACTGGAAAATCCCCATGTTCCTGCTGCCCGCCTTTCCGGCAAATTACATCGTTACAAAATCA
AGCTACGCAGTGCTGGGTATCGTCTGGTCTATCAGGTCGAGGACGAGCAGGTTGTCGTTCTTGTGATCGCAGTAGGCAGA
CGTGATGGCGATGAGGTTTATCATCAGGCTAACCGGCGCTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8297.11 Da        Isoelectric Point: 4.4013

>AT202275 WP_032934863.1 NZ_CP074529:c8365-8147 [Klebsiella pneumoniae]
VKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADENRDW

Download         Length: 219 bp

>AT202275 NZ_CP099364:c4281380-4281132 [Citrobacter braakii]
ATGCCATTTCATATCCTGACCAAAACCGCTGCCAGCATCACCGACCTGAAACGTAACCCAATGGGGACGGTCAACGCCGG
GGAGGGTGAAGCGGTGGCGATTCTGAACCGCAATGAGCCAGCGTTTTACTGCGTGCCGCCTGCGGTTTATGCGTATCTGC
TGGAGCTGGCAGAAGACGCCGAGCTGAACCGTATCTCCGACGAACGCGAAGGCGGAAAACGAATAAAGGTATCTTTAAAT
GACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE27

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE25

References