Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5367705..5368330 Replicon chromosome
Accession NZ_CP074528
Organism Klebsiella pneumoniae strain S14_CRE14

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KHV96_RS26170 Protein ID WP_002882817.1
Coordinates 5367705..5368088 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KHV96_RS26175 Protein ID WP_004150355.1
Coordinates 5368088..5368330 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV96_RS26155 (5365071) 5365071..5365973 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KHV96_RS26160 (5365970) 5365970..5366605 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KHV96_RS26165 (5366602) 5366602..5367531 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KHV96_RS26170 (5367705) 5367705..5368088 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KHV96_RS26175 (5368088) 5368088..5368330 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KHV96_RS26180 (5368535) 5368535..5369452 + 918 WP_004181612.1 alpha/beta hydrolase -
KHV96_RS26185 (5369466) 5369466..5370407 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KHV96_RS26190 (5370452) 5370452..5370889 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KHV96_RS26195 (5370886) 5370886..5371746 - 861 WP_004146232.1 virulence factor BrkB family protein -
KHV96_RS26200 (5371740) 5371740..5372339 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T202274 WP_002882817.1 NZ_CP074528:c5368088-5367705 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T202274 NZ_CP099364:c4190677-4190390 [Citrobacter braakii]
ATGCCAATGGAGTTTGAGTGGGATGCAAACAAGGCACAAAGTAACCACCGTAAACATGGTGTTCGCTTTGAGGACGCTAT
ATTGGTTTTCGACGATCCCCAACACCTGTCGCGGCAGGATCGGTATGAGAACGGTGAGTATCGCTGGCAGACCATCGGTC
TTGTTCATGGCATTGTGGTTATTCTGGTTGCCCATAGCGTTAGGTTTGAAAGTGGCTTTGAAGTCATTCGTATCATCAGC
GCCAGAAAGGCAGATCGTAAAGAGAGGAGTCGTTATGAGCATAGTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT202274 WP_004150355.1 NZ_CP074528:c5368330-5368088 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT202274 NZ_CP099364:c4190403-4190101 [Citrobacter braakii]
ATGAGCATAGTTAAACATAAACGCGGCAGCGTATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
TAAACCGGACGATGATATCGATTACAGCGACATTCCCGTGACCGAAGATAAGCAGTGGTCAGAGGCCATTCGCGGTAAGT
TTTTTCGTCCGTTAAAAACGCAGGCGTCGGTGCGCATTGATGCCGATGTGATGGAATGGCTGAAGCGCCCCGGGAAGGGT
TACCAGACGCGTCTCAATGCCATTTTGCGCGAAGCGATGCTGCGCGAGCAAAATAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References