Detailed information of TA system TA202273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 5054597..5056278
• Replicon: chromosome
• Accession: NZ_CP074528
• Organism: Klebsiella pneumoniae strain S14_CRE14

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: KHV96_RS24730
• Protein ID: WP_004178299.1
• Coordinates: 5054597..5055514 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: KHV96_RS24735
• Protein ID: WP_004177809.1
• Coordinates: 5055520..5056278 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHV96_RS24710 (5049903)	5049903..5051423	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
KHV96_RS24715 (5051420)	5051420..5052418	+	999	WP_002885172.1	ribose ABC transporter permease	-
KHV96_RS24720 (5052449)	5052449..5053390	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
KHV96_RS24725 (5053465)	5053465..5054472	+	1008	WP_004178300.1	carbohydrate kinase family protein	-
KHV96_RS24730 (5054597)	5054597..5055514	-	918	WP_004178299.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
KHV96_RS24735 (5055520)	5055520..5056278	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
KHV96_RS24740 (5056364)	5056364..5057092	-	729	WP_004178298.1	response regulator transcription factor	-
KHV96_RS24745 (5057365)	5057365..5059512	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
KHV96_RS24750 (5059509)	5059509..5059652	-	144	WP_022631365.1	hypothetical protein	-
KHV96_RS24755 (5060090)	5060090..5060776	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34649.06 Da
• Isoelectric Point: 5.4707

>T202273 WP_004178299.1 NZ_CP074528:c5055514-5054597 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPSQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T202273 NZ_CP099364:3656460-3656678 [Citrobacter braakii]
ATGTCCGATAAACCATTAACTAAAACTGATTATTTGATGCGCCTGCGACGTTGTCAGACAATTGACACACTTGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCACCGCCTTGCTGAAT
TGACCATGAATAAGCTCTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT202273 WP_004177809.1 NZ_CP074528:c5056278-5055520 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT202273 NZ_CP099364:3656058-3656432 [Citrobacter braakii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCCACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGGTCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: A6TH05