Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4787957..4788767 Replicon chromosome
Accession NZ_CP074528
Organism Klebsiella pneumoniae strain S14_CRE14

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KHV96_RS23485 Protein ID WP_004178461.1
Coordinates 4787957..4788490 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KHV96_RS23490 Protein ID WP_002887278.1
Coordinates 4788501..4788767 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV96_RS23480 (4786788) 4786788..4787909 + 1122 WP_002887282.1 cupin domain-containing protein -
KHV96_RS23485 (4787957) 4787957..4788490 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KHV96_RS23490 (4788501) 4788501..4788767 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KHV96_RS23495 (4788870) 4788870..4790303 - 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
KHV96_RS23500 (4790293) 4790293..4790976 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KHV96_RS23505 (4791148) 4791148..4792533 + 1386 WP_004178457.1 efflux transporter outer membrane subunit -
KHV96_RS23510 (4792551) 4792551..4792895 + 345 WP_004197273.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T202271 WP_004178461.1 NZ_CP074528:c4788490-4787957 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T202271 NZ_CP099364:2358609-2358797 [Citrobacter braakii]
GTGAAGCAAAGTGAGTTCAGACGCTGGCTTGAATCTCAGGGAGTGGCTGTTACAAACGGCTCAAATCATCTGAAGTTGAG
GTACCAGGGAAGGCGTAGCGTTATGCCCAGGCATCCTGGTGATGAAATAAAAGAAGCCTTGCGAAAATCGATCCTGAAGC
AGCTTGGTTTACAGTCATCATCAATATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT202271 WP_002887278.1 NZ_CP074528:c4788767-4788501 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT202271 NZ_CP099364:2358829-2359245 [Citrobacter braakii]
ATGCGTTATCCCGTTAATCTAATCCCCTCCGTGGAAGGTGGATTCGTGGTTTCTTTCCCGGATATTCCTGAAGCATTAAC
CCAGGGCGAAACCCGTCATGGGGCTTTGCAGGCTGCGCAGTCTGCATTGGTGACCGCTTTTGAATTCTATTTCGACGATA
ACGAACCGATTCCTTTACCTTCGGCGGTTGGCACTGAAGGTGACTATGTGGAAATCCCCCTGAGCATTGCGTCGAAGGTT
CTTTTGTTAAATGCCTTTCTTGAATCAAAAATCACCCAGCAGGAGCTGGCAAACAGGATCGGCAGGCCTAAGCAGGAAAT
TACCCGTTTGTTCGATTTAAAGCACGCGACAAAAATTGATGCTGTGCAAATAGCTGCACGCGCCTTAGGCAAGGAGCTGT
CAGTAACAATGTTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References