Detailed information of TA system TA202268

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2739600..2741188
• Replicon: chromosome
• Accession: NZ_CP074528
• Organism: Klebsiella pneumoniae strain S14_CRE14

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: KHV96_RS13450
• Protein ID: WP_004179815.1
• Coordinates: 2739600..2740922 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: KHV96_RS13455
• Protein ID: WP_004176198.1
• Coordinates: 2740922..2741188 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHV96_RS13435 (2734656)	2734656..2737157	+	2502	WP_020323802.1	fimbria/pilus outer membrane usher protein	-
KHV96_RS13440 (2737145)	2737145..2738149	+	1005	WP_004179822.1	fimbrial protein	-
KHV96_RS13445 (2738291)	2738291..2739583	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
KHV96_RS13450 (2739600)	2739600..2740922	-	1323	WP_004179815.1	type II toxin-antitoxin system HipA family toxin	Toxin
KHV96_RS13455 (2740922)	2740922..2741188	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KHV96_RS13460 (2741454)	2741454..2741780	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
KHV96_RS13465 (2741777)	2741777..2742277	-	501	WP_004179811.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
KHV96_RS13470 (2742255)	2742255..2743634	-	1380	WP_004179808.1	nucleobase:cation symporter-2 family protein	-
KHV96_RS13475 (2743980)	2743980..2745134	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
KHV96_RS13480 (2745115)	2745115..2746038	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49152.42 Da
• Isoelectric Point: 7.2850

>T202268 WP_004179815.1 NZ_CP074528:c2740922-2739600 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTHQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T202268 NZ_CP099364:c57382-57041 [Citrobacter braakii]
ATGAAATCTCTACCTGCAACAACTTCGCGGACGGCAAAGTCCTGCCTGTCGCCTGTGGCTGTCTGGCAAATGCTGCTTAC
CCGTCTGCTCGAAAAACACTATGGCCTGACGCTAAACGACACCCCGTTCAGTGATGAAACTGTGATTCAGGAACACATTG
ATGCCGGTATCACGCTGGCTGATGCCATTAATTTTCTGGTGGAGAAATATGAGCTGGTTCGAATAGATCGCAAAGGATTT
AGCTGGCAGGAACAATCCCCTTATCTCCGGGCTGTAGATATTCTGCGAGCGCGGCAGGCAACTGGCCTGTTACGGCAAAG
CCGTAATAACGCAGTAGGGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT202268 WP_004176198.1 NZ_CP074528:c2741188-2740922 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT202268 NZ_CP099364:c57720-57403 [Citrobacter braakii]
ATGAGTAACATCACATGGGGCCTGCAGCGGGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGGAACCAGCT
GCATTATTTAGCTGATCGGGCCAGTATCACCGGTAAATTCAGTGACGCCGAATGCCGGAAGCTGGATGAAACTTTCCCGC
ATTTTATCAGCCGGATGGAATCCATGCTGACCACGGGTGAAATGAATCCTCGCCATGCCCACTGCGTCATCCTGTACCAC
AATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGCCATTTACCCTACTCAGCTTTAA