Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 690758..691437 Replicon chromosome
Accession NZ_CP074528
Organism Klebsiella pneumoniae strain S14_CRE14

Toxin (Protein)


Gene name ykfI Uniprot ID A0A0C7K7A4
Locus tag KHV96_RS03390 Protein ID WP_020324801.1
Coordinates 691096..691437 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A0C7KEL2
Locus tag KHV96_RS03385 Protein ID WP_020324792.1
Coordinates 690758..691075 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV96_RS03360 (685972) 685972..689124 + 3153 WP_022631414.1 AIDA repeat-containing protein -
KHV96_RS03365 (689217) 689217..689456 + 240 WP_020324820.1 DUF905 domain-containing protein -
KHV96_RS03370 (689559) 689559..690017 + 459 WP_020324813.1 antirestriction protein -
KHV96_RS03375 (690033) 690033..690509 + 477 WP_020324797.1 RadC family protein -
KHV96_RS03380 (690518) 690518..690745 + 228 WP_020324796.1 DUF987 domain-containing protein -
KHV96_RS03385 (690758) 690758..691075 + 318 WP_020324792.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KHV96_RS03390 (691096) 691096..691437 + 342 WP_020324801.1 TA system toxin CbtA family protein Toxin
KHV96_RS03395 (691553) 691553..692386 + 834 WP_020324805.1 DUF4942 domain-containing protein -
KHV96_RS03405 (692689) 692689..693195 + 507 WP_002917636.1 G/U mismatch-specific DNA glycosylase -
KHV96_RS03410 (693295) 693295..695136 - 1842 WP_004174339.1 RNA polymerase sigma factor RpoD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimA / fimI / fimC / fimD / fimD / fimF / fimG 643971..692386 48415


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-111)

Antitoxin

Antitoxin CbeA

(5-103)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12818.91 Da        Isoelectric Point: 9.1484

>T202258 WP_020324801.1 NZ_CP074528:691096-691437 [Klebsiella pneumoniae]
MKTLPAIISRAAKPCLSPVAVWQMLLTRLLEKHYGLTLNDTPFCDETVIQEHIDAGITLADAVNFLVEKYGLVRIDRNGF
VWQEQSPYLRAVDILRARQATGLLRQSRNNAIR

Download         Length: 342 bp

>T202258 NZ_CP099358:3293221-3293439 [Escherichia fergusonii]
ATGTCCGATAAGCCTTTAACCAAAACTGATTATTTGATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAACGTGT
CATCGAAAAAAATAAATACGAACTCTCTGACAATGAACTGGCGGTATTTTATTCAGCAGCTGATCACCGTCTCGCTGAAT
TGACCATGAATAAACTTTACGACAAGATCCCCTCCTCCGTGTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11915.51 Da        Isoelectric Point: 6.6246

>AT202258 WP_020324792.1 NZ_CP074528:690758-691075 [Klebsiella pneumoniae]
MSNITWGLQRDTTPRLGARLVQEGNQLHYLADRASMTGKFSDAECRKLDETFPHFISQMESMLITGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYVYIAVYPIQR

Download         Length: 318 bp

>AT202258 NZ_CP099358:3292820-3293194 [Escherichia fergusonii]
ATGGACGAATACTCACCAAAACGACATGATATCGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAATCTTGAAGAGAGCAACCATGGCTGGGTTAACGACCCAACCTCGGCTATCAATCTGCAGCTCAACGAACTGATTGAGC
ATATTGCAACCTTCGCACTTAATTACAAAATTAAGTATAATGAGGACAATAAGCTCATAGAGCAGATCGATGAATATCTG
GATGACACTTTTATGTTGTTCAGTAGTTATGGCATTAATACACAGGATTTACAGAAATGGCGAAAATCTGGAAACAGGCT
CTTCCGCTGTTTTGTTAACGCCACCAGGGCGAACCCTGTTAGTTTGTCGTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C7K7A4

Antitoxin

Source ID Structure
AlphaFold DB A0A0C7KEL2

References