Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 43201..43844 | Replicon | plasmid pCRE12_2 |
| Accession | NZ_CP074525 | ||
| Organism | Klebsiella pneumoniae strain S12_CRE12 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | D8L2J9 |
| Locus tag | KHV95_RS28595 | Protein ID | WP_001044770.1 |
| Coordinates | 43428..43844 (+) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | D5KTK7 |
| Locus tag | KHV95_RS28590 | Protein ID | WP_001261282.1 |
| Coordinates | 43201..43431 (+) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KHV95_RS28555 (KHV95_28465) | 38918..39673 | - | 756 | WP_012539983.1 | replication initiation protein RepE | - |
| KHV95_RS28560 (KHV95_28470) | 40304..40417 | + | 114 | WP_032072094.1 | hypothetical protein | - |
| KHV95_RS28565 (KHV95_28475) | 40546..40803 | + | 258 | WP_032408936.1 | hypothetical protein | - |
| KHV95_RS28570 (KHV95_28480) | 40861..41646 | - | 786 | WP_022644886.1 | site-specific integrase | - |
| KHV95_RS28575 (KHV95_28485) | 41649..42287 | - | 639 | WP_022644887.1 | hypothetical protein | - |
| KHV95_RS28580 (KHV95_28490) | 42336..42656 | - | 321 | WP_032072060.1 | hypothetical protein | - |
| KHV95_RS28585 (KHV95_28495) | 42784..43244 | - | 461 | Protein_50 | hypothetical protein | - |
| KHV95_RS28590 (KHV95_28500) | 43201..43431 | + | 231 | WP_001261282.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| KHV95_RS28595 (KHV95_28505) | 43428..43844 | + | 417 | WP_001044770.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| KHV95_RS28600 (KHV95_28510) | 43918..44136 | + | 219 | Protein_53 | AAA family ATPase | - |
| KHV95_RS28605 (KHV95_28515) | 44201..44905 | + | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| KHV95_RS28610 (KHV95_28520) | 44958..45725 | - | 768 | Protein_55 | class 1 integron integrase IntI1 | - |
| KHV95_RS28615 (KHV95_28525) | 45881..46354 | + | 474 | WP_004201280.1 | trimethoprim-resistant dihydrofolate reductase DfrA14 | - |
| KHV95_RS28620 | 46575..46691 | + | 117 | Protein_57 | plasmid mobilization relaxosome protein MobC | - |
| KHV95_RS28625 | 46801..46866 | + | 66 | Protein_58 | EAL domain-containing protein | - |
| KHV95_RS28630 (KHV95_28535) | 46984..47748 | + | 765 | WP_001389365.1 | IS6-like element IS6100 family transposase | - |
| KHV95_RS28635 (KHV95_28540) | 47888..47953 | - | 66 | Protein_60 | EAL domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | blaKPC-3 / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib | - | 1..71941 | 71941 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15106.52 Da Isoelectric Point: 7.1084
>T202256 WP_001044770.1 NZ_CP074525:43428-43844 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
Download Length: 417 bp
>T202256 NZ_CP099358:c2804211-2803957 [Escherichia fergusonii]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACTCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACTCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8756.83 Da Isoelectric Point: 4.4692
>AT202256 WP_001261282.1 NZ_CP074525:43201-43431 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
Download Length: 231 bp
>AT202256 NZ_CP099358:c2804459-2804208 [Escherichia fergusonii]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAACGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCCCTGGAAGAGACGGCTTATC
TATTGCGTTCCCCCGCTAATGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAACGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCCCTGGAAGAGACGGCTTATC
TATTGCGTTCCCCCGCTAATGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3GYM2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3GZG3 |