Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5405649..5406274 Replicon chromosome
Accession NZ_CP074523
Organism Klebsiella pneumoniae strain S12_CRE12

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KHV95_RS26850 Protein ID WP_002882817.1
Coordinates 5405649..5406032 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KHV95_RS26855 Protein ID WP_004150355.1
Coordinates 5406032..5406274 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV95_RS26835 (KHV95_26775) 5403015..5403917 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KHV95_RS26840 (KHV95_26780) 5403914..5404549 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KHV95_RS26845 (KHV95_26785) 5404546..5405475 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KHV95_RS26850 (KHV95_26790) 5405649..5406032 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KHV95_RS26855 (KHV95_26795) 5406032..5406274 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KHV95_RS26860 (KHV95_26800) 5406479..5407396 + 918 WP_002882812.1 alpha/beta hydrolase -
KHV95_RS26865 (KHV95_26805) 5407410..5408351 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KHV95_RS26870 (KHV95_26810) 5408396..5408833 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KHV95_RS26875 (KHV95_26815) 5408830..5409690 - 861 WP_002882807.1 virulence factor BrkB family protein -
KHV95_RS26880 (KHV95_26820) 5409684..5410283 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T202254 WP_002882817.1 NZ_CP074523:c5406032-5405649 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T202254 NZ_CP099358:1909605-1909708 [Escherichia fergusonii]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT202254 WP_004150355.1 NZ_CP074523:c5406274-5406032 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT202254 NZ_CP099358:c1909746-1909478 [Escherichia fergusonii]
AAAGTCAGCGAATGGAATATTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References