Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4817070..4817880 Replicon chromosome
Accession NZ_CP074523
Organism Klebsiella pneumoniae strain S12_CRE12

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag KHV95_RS24025 Protein ID WP_002887280.1
Coordinates 4817070..4817603 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KHV95_RS24030 Protein ID WP_002887278.1
Coordinates 4817614..4817880 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV95_RS24020 (KHV95_23970) 4815901..4817022 + 1122 WP_002887282.1 cupin domain-containing protein -
KHV95_RS24025 (KHV95_23975) 4817070..4817603 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
KHV95_RS24030 (KHV95_23980) 4817614..4817880 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KHV95_RS24035 (KHV95_23985) 4817983..4819416 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
KHV95_RS24040 (KHV95_23990) 4819406..4820089 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KHV95_RS24045 (KHV95_23995) 4820261..4821646 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
KHV95_RS24050 (KHV95_24000) 4821664..4822008 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T202251 WP_002887280.1 NZ_CP074523:c4817603-4817070 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T202251 NZ_CP099358:870370-870522 [Escherichia fergusonii]
ATGCTGACAAAATATGCCCTTGTGGCAATCATCGTACTGTGTTGTACAGTACTGGGATTCACGCTGATGGTAGGCGACTC
GTTGTGTGAGTTGAGTATCAGAGAACGTGGTATGGAGTTTAAGGCAGTTCTCGCTTACGAATCGAAGAAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT202251 WP_002887278.1 NZ_CP074523:c4817880-4817614 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT202251 NZ_CP099358:c870328-870276 [Escherichia fergusonii]
TTAGGTTCGAACGCTGGCCTCAGGTTGATAGAAATATCGCCTGGGGCTTTTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References