Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 121427..122079 Replicon plasmid pCRE10_1
Accession NZ_CP074520
Organism Klebsiella pneumoniae strain S10_CRE10

Toxin (Protein)


Gene name vagD Uniprot ID A0A8A2Q2K1
Locus tag KHV94_RS25855 Protein ID WP_017901321.1
Coordinates 121427..121852 (-) Length 142 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A853H7M9
Locus tag KHV94_RS25860 Protein ID WP_001261275.1
Coordinates 121849..122079 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV94_RS25820 (KHV94_25760) 117136..117252 + 117 Protein_123 transposase -
KHV94_RS25825 (KHV94_25765) 117377..117547 - 171 Protein_124 LysR family transcriptional regulator -
KHV94_RS25830 117558..117785 + 228 Protein_125 IS3 family transposase -
KHV94_RS25835 (KHV94_25775) 117877..119433 - 1557 WP_025999314.1 sensor domain-containing diguanylate cyclase -
KHV94_RS25840 119620..119793 - 174 Protein_127 nuclease -
KHV94_RS25845 (KHV94_25780) 119846..120382 - 537 Protein_128 integrase core domain-containing protein -
KHV94_RS25850 (KHV94_25785) 120441..121409 - 969 WP_102097429.1 IS5 family transposase -
KHV94_RS25855 (KHV94_25790) 121427..121852 - 426 WP_017901321.1 type II toxin-antitoxin system VapC family toxin Toxin
KHV94_RS25860 (KHV94_25795) 121849..122079 - 231 WP_001261275.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KHV94_RS25865 (KHV94_25800) 122332..124908 - 2577 WP_017901322.1 nuclease domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..226793 226793
- flank IS/Tn - - 120441..121409 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15412.91 Da        Isoelectric Point: 7.1364

>T202236 WP_017901321.1 NZ_CP074520:c121852-121427 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVGFVE

Download         Length: 426 bp

>T202236 NZ_CP099351:393600-393773 [Escherichia marmotae]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTCTCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGCCTGCCCGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8707.72 Da        Isoelectric Point: 4.3410

>AT202236 WP_001261275.1 NZ_CP074520:c122079-121849 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDP

Download         Length: 231 bp

>AT202236 NZ_CP099351:393277-393573 [Escherichia marmotae]
ATGGAAGGTATAAACAGGTTCAAAACTTACGTTGTTTCTTTTGATTATCCATCATCTTATTACTCAGTTTTCTTAAGGTT
AAGGTCGTTAATGTATGATATGGATTTCTCCTCTATAGTTGCTGATGAATATGGAATACCACGACAATTGAATGAGAATG
CTTTCGCAATAACGACATCATTAGCCGCAAGTGAAATTGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCAGATATT
GATTTTGACCTCAACATTATGATAGTCGATGACTATTTCCGTCAGTTCTACAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8A2Q2K1

Antitoxin

Source ID Structure
AlphaFold DB A0A853H7M9

References