Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5080749..5081374 Replicon chromosome
Accession NZ_CP074519
Organism Klebsiella pneumoniae strain S10_CRE10

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KHV94_RS24840 Protein ID WP_002882817.1
Coordinates 5080749..5081132 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KHV94_RS24845 Protein ID WP_004150355.1
Coordinates 5081132..5081374 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV94_RS24825 (5078115) 5078115..5079017 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KHV94_RS24830 (5079014) 5079014..5079649 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KHV94_RS24835 (5079646) 5079646..5080575 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KHV94_RS24840 (5080749) 5080749..5081132 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KHV94_RS24845 (5081132) 5081132..5081374 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KHV94_RS24850 (5081579) 5081579..5082496 + 918 WP_023302328.1 alpha/beta hydrolase -
KHV94_RS24855 (5082510) 5082510..5083451 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KHV94_RS24860 (5083496) 5083496..5083933 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KHV94_RS24865 (5083930) 5083930..5084790 - 861 WP_004146232.1 virulence factor BrkB family protein -
KHV94_RS24870 (5084784) 5084784..5085383 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T202233 WP_002882817.1 NZ_CP074519:c5081132-5080749 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T202233 NZ_CP099349:4234579-4234977 [Escherichia fergusonii]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCCGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATTTATGGGGCAGAAAAAAGCCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGCTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCCGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCTGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT202233 WP_004150355.1 NZ_CP074519:c5081374-5081132 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT202233 NZ_CP099349:4234352-4234579 [Escherichia fergusonii]
ATGGAAACCACTGTATTTTTAAGTAATCGCAGTCAGGCTGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGACGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References