Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4511931..4512741 Replicon chromosome
Accession NZ_CP074519
Organism Klebsiella pneumoniae strain S10_CRE10

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KHV94_RS22170 Protein ID WP_004178461.1
Coordinates 4511931..4512464 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KHV94_RS22175 Protein ID WP_002887278.1
Coordinates 4512475..4512741 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV94_RS22165 (4510762) 4510762..4511883 + 1122 WP_012737335.1 cupin domain-containing protein -
KHV94_RS22170 (4511931) 4511931..4512464 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KHV94_RS22175 (4512475) 4512475..4512741 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KHV94_RS22180 (4512844) 4512844..4514277 - 1434 WP_029498528.1 Cu(+)/Ag(+) sensor histidine kinase -
KHV94_RS22185 (4514267) 4514267..4514950 - 684 WP_023302433.1 copper response regulator transcription factor CusR -
KHV94_RS22190 (4515122) 4515122..4516507 + 1386 WP_023302432.1 efflux transporter outer membrane subunit -
KHV94_RS22195 (4516525) 4516525..4516869 + 345 WP_004214146.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T202230 WP_004178461.1 NZ_CP074519:c4512464-4511931 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T202230 NZ_CP099349:c3525084-3524932 [Escherichia fergusonii]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT202230 WP_002887278.1 NZ_CP074519:c4512741-4512475 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT202230 NZ_CP099349:3525133-3525190 [Escherichia fergusonii]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References