Detailed information of TA system TA202228

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2574413..2576001
• Replicon: chromosome
• Accession: NZ_CP074519
• Organism: Klebsiella pneumoniae strain S10_CRE10

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A486S9S0
• Locus tag: KHV94_RS12725
• Protein ID: WP_023301940.1
• Coordinates: 2574413..2575735 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: KHV94_RS12730
• Protein ID: WP_024622638.1
• Coordinates: 2575735..2576001 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHV94_RS12710 (2569469)	2569469..2571970	+	2502	WP_270104253.1	fimbria/pilus outer membrane usher protein	-
KHV94_RS12715 (2571958)	2571958..2572962	+	1005	WP_023301939.1	fimbrial protein	-
KHV94_RS12720 (2573104)	2573104..2574396	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
KHV94_RS12725 (2574413)	2574413..2575735	-	1323	WP_023301940.1	type II toxin-antitoxin system HipA family toxin	Toxin
KHV94_RS12730 (2575735)	2575735..2576001	-	267	WP_024622638.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KHV94_RS12735 (2576266)	2576266..2576592	-	327	WP_004184023.1	hydroxyisourate hydrolase	-
KHV94_RS12740 (2576589)	2576589..2577089	-	501	WP_004184022.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
KHV94_RS12745 (2577067)	2577067..2578446	-	1380	WP_023301941.1	nucleobase:cation symporter-2 family protein	-
KHV94_RS12750 (2578792)	2578792..2579946	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
KHV94_RS12755 (2579927)	2579927..2580850	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49185.49 Da
• Isoelectric Point: 7.5358

>T202228 WP_023301940.1 NZ_CP074519:c2575735-2574413 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T202228 NZ_CP099349:3394264-3394554 [Escherichia fergusonii]
ATGAACTATGAACTGGCTTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGGGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTACGGGATTTGCCGGACTGCTACAAAATTA
AGCTTCGCACACTGGGATATCGCCGGGTCTATCAGGTCAATGATAAAGAGTTACTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9924.30 Da
• Isoelectric Point: 8.5912

>AT202228 WP_024622638.1 NZ_CP074519:c2576001-2575735 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYMKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT202228 NZ_CP099349:3394032-3394274 [Escherichia fergusonii]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCAGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAGGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Structures

Toxin

• AlphaFold DB: A0A486S9S0