202216

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4959610..4960396 Replicon chromosome
Accession NZ_CP074449
Organism Klebsiella michiganensis strain S4_CRE4

Toxin (Protein)


Gene name yeeV Uniprot ID A0A097QYR4
Locus tag KHV91_RS23325 Protein ID WP_025799003.1
Coordinates 4959610..4959987 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A097QYU1
Locus tag KHV91_RS23330 Protein ID WP_025799001.1
Coordinates 4960037..4960396 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV91_RS23295 (KHV91_23240) 4956422..4957084 + 663 WP_064404271.1 TetR/AcrR family transcriptional regulator -
KHV91_RS23300 (KHV91_23245) 4957154..4957660 - 507 WP_009651862.1 G/U mismatch-specific DNA glycosylase -
KHV91_RS23310 (KHV91_23255) 4957963..4958808 - 846 WP_049016442.1 DUF4942 domain-containing protein -
KHV91_RS23315 (KHV91_23260) 4958890..4959093 - 204 WP_025799007.1 DUF957 domain-containing protein -
KHV91_RS23320 (KHV91_23265) 4959122..4959613 - 492 WP_045888216.1 DUF5983 family protein -
KHV91_RS23325 (KHV91_23270) 4959610..4959987 - 378 WP_025799003.1 TA system toxin CbtA family protein Toxin
KHV91_RS23330 (KHV91_23275) 4960037..4960396 - 360 WP_025799001.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KHV91_RS23335 (KHV91_23280) 4960420..4960641 - 222 WP_045888217.1 DUF987 domain-containing protein -
KHV91_RS23340 (KHV91_23285) 4960655..4961134 - 480 WP_025798999.1 DNA repair protein RadC -
KHV91_RS23345 (KHV91_23290) 4961146..4961577 - 432 WP_088903392.1 antirestriction protein -
KHV91_RS23350 (KHV91_23295) 4961800..4964952 - 3153 WP_049015608.1 autotransporter domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(15-114)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13993.97 Da        Isoelectric Point: 6.8515

>T202216 WP_025799003.1 NZ_CP074449:c4959987-4959610 [Klebsiella michiganensis]
MQTQPVPPTQEASPRPSPVEIWLRLLSHLLDRHYGLTLNDTPFCNDGVIQEHIDAGISLCDAVNFIVEKYALIRTDRHGF
SAETQSPLISSIDILRARKACGLMARNGYRLVTDITTGKYSEVTR

Download         Length: 378 bp

>T202216 NZ_CP099347:3257-3523 [Escherichia marmotae]
TTGGTCTGGACGATTAACTATTCCGACCGGGCGCTCAAATCGTTACGTAAGATGGACAAGCAGAACGCGCGTCGGATTGT
GGACTTTATGGATTTACGTATTGCTGTTGCCGCCGATCCTCGTCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGGGAGT
TCTGGCGCTATCGTGTGGGGGATTATCGCATTCTGTGTGAGATCCGGGATGACGAGCTGGTTATCCTTGCGGCCACCATA
GGCCATCGCCGTGAAGTCTACGACTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13249.13 Da        Isoelectric Point: 6.9556

>AT202216 WP_025799001.1 NZ_CP074449:c4960396-4960037 [Klebsiella michiganensis]
MSNKIPPVNHNIAEPWWGLKRDITPCFGARLVQEGNRLHYLADRASITGQFSDADLRHLDQAFPLLLKQLELMLTSGELN
PRHQHGVTLYAKGLTCNADTLGSCGYVYIAIYPTPATTE

Download         Length: 360 bp

>AT202216 NZ_CP099347:3049-3273 [Escherichia marmotae]
ATGTTGGCTATCAGGCTGTCTGCTGAAATTGAGTCCCGTCTTGATTCACTGGCGAAACAAACCGGCAGAACAAAGACGTT
CTATGCGCGGGAAGCGATACTGGCACACCTGGAAGACCTAGAAGATTACTATCTTTCAGCAGAAACTGCGGCACGCGTTC
GCCGTGGCGATGAACCTGTGCATTCGTCTGAAGATGTGAGGAAGTCACTTGGTCTGGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A097QYR4

Antitoxin

Source ID Structure
AlphaFold DB A0A097QYU1

References