Detailed information of TA system TA202215

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 4803370..4804027
• Replicon: chromosome
• Accession: NZ_CP074449
• Organism: Klebsiella michiganensis strain S4_CRE4

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: KHV91_RS22545
• Protein ID: WP_064404221.1
• Coordinates: 4803370..4803780 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: H3N295
• Locus tag: KHV91_RS22550
• Protein ID: WP_004124953.1
• Coordinates: 4803761..4804027 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHV91_RS22525 (KHV91_22470)	4799347..4801080	-	1734	WP_032751182.1	single-stranded-DNA-specific exonuclease RecJ	-
KHV91_RS22530 (KHV91_22475)	4801086..4801799	-	714	WP_004854054.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
KHV91_RS22535 (KHV91_22480)	4801822..4802718	-	897	WP_004105555.1	site-specific tyrosine recombinase XerD	-
KHV91_RS22540 (KHV91_22485)	4802840..4803361	+	522	WP_014226792.1	flavodoxin FldB	-
KHV91_RS22545 (KHV91_22490)	4803370..4803780	-	411	WP_064404221.1	protein YgfX	Toxin
KHV91_RS22550 (KHV91_22495)	4803761..4804027	-	267	WP_004124953.1	FAD assembly factor SdhE	Antitoxin
KHV91_RS22555 (KHV91_22500)	4804272..4805255	+	984	WP_014226793.1	tRNA-modifying protein YgfZ	-
KHV91_RS22560 (KHV91_22505)	4805449..4808247	+	2799	WP_014226794.1	transcriptional regulator DagR	-
KHV91_RS22565 (KHV91_22510)	4808368..4808793	+	426	WP_004854067.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16122.97 Da
• Isoelectric Point: 10.9455

>T202215 WP_064404221.1 NZ_CP074449:c4803780-4803370 [Klebsiella michiganensis]
VVLWQSDLRISWRAQWFSLLMHGVVAALVLLMPWPLSYTPLWLILLSLVVFDCVRSQRRIHARQGEIKLLIDSRLRWQKA
EWDIIGTPWVINSGMLLRLRNTENQRTQHLWVAADSMDSGEWRDLRRLVLLKPTQE

Nucleotide

• Length: 411 bp

>T202215 NZ_CP099346:10009-10284 [Escherichia marmotae]
TTGTCGAAGTGGAATATTAAGTTCACCAGGAACGGCGCTGATGACCTTAACTACTGGCGTGATACTGACAAAAAGAAGTT
TGAGCGTATCCGGCAGCTGTTGCACAGCATCGAGGCTGATCCGCTGACAGGTATCGGAAAACCTGAACGGCTGCGGCACC
ACAAAGATCCGGCGCTCTATTCTCGCCGGATTGATCAGAGCCACCGGCTGGTCTACTCAATCCAGAACGGAGAAATCATC
ATCTACGCAGCCCGTTATCACTATGGTGAAAAATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10411.88 Da
• Isoelectric Point: 5.1296

>AT202215 WP_004124953.1 NZ_CP074449:c4804027-4803761 [Klebsiella michiganensis]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDGDKALFIRLLENDDPDLFNWLMNHGEPADAELQRMVKLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT202215 NZ_CP099346:9783-10028 [Escherichia marmotae]
ATGCATGCAATCAATTTTACGGATGCCCGTAAACATTTTGCCGACACGATGAAGCGCGTCACTGATGATGCTGAACCCGT
ACGTGTCATGCGTCGTAACGCGCCTGATATTGTTATGATCGATGCGGGAGAGTATGAGGCGTTAATTGAAACGGTCTATC
TGTTCAGCAATCCGGCGAATGCCGCACATATCAATGAGTCAATGAAACAGGCGGAGCGCGGCGAGTTTGTCGAAGTGGAA
TATTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3H3L9