Detailed information of TA system TA202214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 3631120..3631710
• Replicon: chromosome
• Accession: NZ_CP074449
• Organism: Klebsiella michiganensis strain S4_CRE4

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A1F2LYA2
• Locus tag: KHV91_RS17000
• Protein ID: WP_008804165.1
• Coordinates: 3631120..3631452 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: KHV91_RS17005
• Protein ID: WP_064405693.1
• Coordinates: 3631453..3631710 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHV91_RS16975 (KHV91_16935)	3626533..3626790	+	258	WP_014229908.1	histidine kinase	-
KHV91_RS16980 (KHV91_16940)	3626923..3628377	+	1455	WP_014229909.1	AMP nucleosidase	-
KHV91_RS16990 (KHV91_16950)	3629339..3630796	-	1458	WP_025106072.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
KHV91_RS17000 (KHV91_16960)	3631120..3631452	-	333	WP_008804165.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KHV91_RS17005 (KHV91_16965)	3631453..3631710	-	258	WP_064405693.1	hypothetical protein	Antitoxin
KHV91_RS17010 (KHV91_16970)	3631971..3632174	-	204	WP_224243742.1	helix-turn-helix domain-containing protein	-
KHV91_RS17015 (KHV91_16975)	3632431..3634386	+	1956	WP_064405695.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11867.71 Da
• Isoelectric Point: 10.1839

>T202214 WP_008804165.1 NZ_CP074449:c3631452-3631120 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Nucleotide

• Length: 333 bp

>T202214 NZ_CP099345:96828-97310 [Escherichia marmotae]
ATGGAAATAAATGTCACCGCGCCGGCATTGCTGACAGATGAGCATATACTTCAGCCATTTGACTGTGGGAATGAGGTGTT
AAGTAACTGGTTGCGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCCCGCACGTTCGTCATTTGTCTGGAGGATA
CTTTACGTGTTGTGGGGTATTACTCATTAGCTACTGGTTCTGTCACACATGCCGAGCTTGGGCGCAGTTTGCGCCATAAT
ATGCCCAATCCTGTACCTGTTGTTCTTTTAGGGCGTCTGGCTGTCGATGTTTGTACTCAGGGACATGGTTTCGGTAAATG
GCTGCTGAGTGATGCCATTCATCGGGTTGCCAATCTGGCTGAACAGGTTGGCATTAAGGCTGTTATGGTTCATGCAATTG
ATGATGACGCCAGAGTTTTTTATGAGCATTTTGGTTTTGTTCAGTCAGTTGTGGCACCTGATACCTTGTTTTATAAAATT
TGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9452.97 Da
• Isoelectric Point: 4.4607

>AT202214 WP_064405693.1 NZ_CP074449:c3631710-3631453 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSMDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT202214 NZ_CP099345:96547-96837 [Escherichia marmotae]
ATGTCCACTGCAGCAAGCATCCGTAAAACACCGCGAGAAAATCAGATTAACATCCGGGCAACTGATGAGGAAAGAGCCGT
TATTGATTATGCTGCGAGCCTGGTCAATAAGAATCGTACCGATTTCATCATTGAACTGGCTTACCAGGAAGCTAAAAACA
TCATTCTGGATCAACGTTTATTTGTATTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAAGCTCCTGTACAG
AATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Structures

Toxin

• AlphaFold DB: A0A1F2LYA2