202205

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1150266..1150885 Replicon chromosome
Accession NZ_CP074449
Organism Klebsiella michiganensis strain S4_CRE4

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag KHV91_RS05370 Protein ID WP_004099646.1
Coordinates 1150266..1150484 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A0J2I7Z1
Locus tag KHV91_RS05375 Protein ID WP_025107145.1
Coordinates 1150511..1150885 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV91_RS05335 (KHV91_05330) 1146311..1146574 + 264 WP_004848323.1 type B 50S ribosomal protein L31 -
KHV91_RS05340 (KHV91_05335) 1146574..1146714 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
KHV91_RS05345 (KHV91_05340) 1146711..1147409 - 699 WP_048261453.1 GNAT family protein -
KHV91_RS05350 (KHV91_05345) 1147511..1148965 + 1455 WP_064405080.1 PLP-dependent aminotransferase family protein -
KHV91_RS05355 (KHV91_05350) 1148940..1149410 - 471 WP_004848329.1 YlaC family protein -
KHV91_RS05360 1149431..1149565 + 135 WP_224243896.1 hypothetical protein -
KHV91_RS05365 (KHV91_05355) 1149537..1150103 - 567 WP_064357999.1 maltose O-acetyltransferase -
KHV91_RS05370 (KHV91_05360) 1150266..1150484 - 219 WP_004099646.1 HHA domain-containing protein Toxin
KHV91_RS05375 (KHV91_05365) 1150511..1150885 - 375 WP_025107145.1 Hha toxicity modulator TomB Antitoxin
KHV91_RS05380 (KHV91_05370) 1151368..1154514 - 3147 WP_014228207.1 multidrug efflux RND transporter permease subunit AcrB -
KHV91_RS05385 (KHV91_05375) 1154537..1155730 - 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-115)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T202205 WP_004099646.1 NZ_CP074449:c1150484-1150266 [Klebsiella michiganensis]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T202205 NZ_CP099344:879704-879811 [Escherichia marmotae]
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTTGCGGGAATTATCACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14366.15 Da        Isoelectric Point: 4.8989

>AT202205 WP_025107145.1 NZ_CP074449:c1150885-1150511 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNACRENPASLSC

Download         Length: 375 bp

>AT202205 NZ_CP099344:c879644-879589 [Escherichia marmotae]
CAAGATTAGCCCCCGTTCTGTTGTCAGGTTTGACCTCTCAACGTGCGGGGGTTTTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0J2I7Z1

References