Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 468106..468682 Replicon chromosome
Accession NZ_CP074449
Organism Klebsiella michiganensis strain S4_CRE4

Toxin (Protein)


Gene name shpA Uniprot ID A0A1Q8YXR6
Locus tag KHV91_RS02210 Protein ID WP_046878034.1
Coordinates 468106..468393 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0H3H591
Locus tag KHV91_RS02215 Protein ID WP_014227757.1
Coordinates 468380..468682 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV91_RS02180 (KHV91_02180) 463446..463964 - 519 WP_004098240.1 FidL-like protein -
KHV91_RS02185 (KHV91_02185) 463961..464761 - 801 WP_064406613.1 winged helix-turn-helix domain-containing protein -
KHV91_RS02190 (KHV91_02190) 464907..465491 - 585 WP_004098242.1 TetR/AcrR family transcriptional regulator -
KHV91_RS02195 (KHV91_02195) 465639..466421 - 783 WP_064358753.1 NAD(P)H-dependent oxidoreductase -
KHV91_RS02200 (KHV91_02200) 466509..467417 + 909 WP_064375700.1 LysR family transcriptional regulator -
KHV91_RS02205 (KHV91_02205) 467411..467854 + 444 WP_014227755.1 FosA family fosfomycin resistance glutathione transferase -
KHV91_RS02210 (KHV91_02210) 468106..468393 + 288 WP_046878034.1 BrnT family toxin Toxin
KHV91_RS02215 (KHV91_02215) 468380..468682 + 303 WP_014227757.1 BrnA antitoxin family protein Antitoxin
KHV91_RS02220 (KHV91_02220) 468803..470146 - 1344 WP_049101852.1 APC family permease -
KHV91_RS02225 (KHV91_02225) 470143..471054 - 912 WP_049080536.1 acetamidase/formamidase family protein -
KHV91_RS02230 (KHV91_02230) 471511..471846 - 336 WP_064375703.1 endoribonuclease SymE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(38-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11210.69 Da        Isoelectric Point: 7.4687

>T202203 WP_046878034.1 NZ_CP074449:468106-468393 [Klebsiella michiganensis]
MPMEFEWDANKAISNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGIIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Download         Length: 288 bp

>T202203 NZ_CP099344:616833-617111 [Escherichia marmotae]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTAATTGATGCAGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGCGATGAAATTTGGACGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGGCTATCACTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11607.18 Da        Isoelectric Point: 9.4429

>AT202203 WP_014227757.1 NZ_CP074449:468380-468682 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Download         Length: 303 bp

>AT202203 NZ_CP099344:617111-617395 [Escherichia marmotae]
ATGAAAATGGCCAACCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACACCAGAAATGGCGA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGCCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q8YXR6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H591

References