Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 56771..57318 Replicon chromosome
Accession NZ_CP074449
Organism Klebsiella michiganensis strain S4_CRE4

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag KHV91_RS00285 Protein ID WP_014227299.1
Coordinates 56771..57079 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag KHV91_RS00290 Protein ID WP_014227298.1
Coordinates 57082..57318 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV91_RS00260 (KHV91_00260) 52687..52998 - 312 WP_014227304.1 PTS sugar transporter subunit IIB -
KHV91_RS00265 (KHV91_00265) 53293..54234 + 942 WP_025107585.1 LacI family DNA-binding transcriptional regulator -
KHV91_RS00270 (KHV91_00270) 54258..54551 - 294 WP_064357598.1 YicS family protein -
KHV91_RS00275 (KHV91_00275) 54761..55714 + 954 WP_032751828.1 Rpn family recombination-promoting nuclease/putative transposase -
KHV91_RS00280 (KHV91_00280) 55718..56620 - 903 WP_014227300.1 EamA family transporter -
KHV91_RS00285 (KHV91_00285) 56771..57079 - 309 WP_014227299.1 CcdB family protein Toxin
KHV91_RS00290 (KHV91_00290) 57082..57318 - 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
KHV91_RS00295 (KHV91_00295) 57424..58857 - 1434 WP_049083310.1 PTS N-acetylmuramic acid transporter subunit IIBC -
KHV91_RS00300 (KHV91_00300) 58882..59784 - 903 WP_032752002.1 N-acetylmuramic acid 6-phosphate etherase -
KHV91_RS00305 (KHV91_00305) 59945..61111 - 1167 WP_064406020.1 multidrug effflux MFS transporter -
KHV91_RS00310 (KHV91_00310) 61244..62182 - 939 Protein_61 efflux transporter outer membrane subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T202202 WP_014227299.1 NZ_CP074449:c57079-56771 [Klebsiella michiganensis]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

>T202202 NZ_CP099344:584237-585559 [Escherichia marmotae]
ATGCCAAAACTTGTCACGTGGATGAACAACCAGCGGGTAGGCGAGTTGACGAAGCTGACCAACGGTGCCCATACCTTTAA
GTATGCGCCGGAGTGGTTGACCAACCGTTATGCCAGGCCATTGTCGCTTTCGCTGCCATTGCAAAGGGGGAATATCACCT
CTGATGCTGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGCGACCGCATCGTTAAACGTTATCAT
GCTAAATCCAGGCAACCGTTTGATTTATTATCAGAAATAGGGCGAGATAGCGTTGGCGCCGTGACGTTATTACCAGAAGA
CGAAACCGTGACGCGCCCAATAATGGTATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGGGAGGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACTGCGCTG
CTCAGAATGGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCATATCATTAAATTGCCGATTGGTGAAAT
CAGACAGCCCAATGCGACGCTCGATCTCAGTCAGAGTGTTGATAATGAGTATTACTGTCTGCTGCTGGCAAAAGAATTCG
GGTTGAATGTTCCCGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGCTTTGACAGGCGCTGG
AATACTGAACGAACGGTTTTACTTCGCTTGCCGCAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCTGACGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTTCGCGATCGTTATG
ATTTTATGAAATTCCAGGTATTCCAGTGGTTGATTGGCTCGACAGACGGCCATGCGAAAAATTTCTCAGTGTTTATCCAG
GCCGGTGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTATTGGGTGGCGCGGGAATACATAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAGAAAAACGGAAATCGATAAAATATATCCGCGACATT
TTTTGGCGACAGCAAAGGCACTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCC
GCAGCATTGGATAACGTGAAGGCTTCATTACCGGCAGATTTTCCGGAGAACGTGTTGACGGCAATTGAAACCAATGTGTT
GAGGTTGCACGGTCGGTTAAGCAGAGAATATGGAATTAAGTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT202202 WP_014227298.1 NZ_CP074449:c57318-57082 [Klebsiella michiganensis]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT202202 NZ_CP099344:583971-584237 [Escherichia marmotae]
ATGATGAGCTTTCCGATGATCTATAGCCCAACGCAATTAGCAAATGCAATGAAACTGGTTCGTCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATCGGCATAAAACAGGCGACTATTTCCAATTTCGAAAATAACCCTGACAACACCACAC
TAACGACATTTTTTAAGATATTACAGTCACTTAATCTCTCGATGACGCTATGCGACACGAAAAATGACTCGCCAGAATCA
ACCGATCAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References