Detailed information of TA system TA202201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 4431627..4432243
• Replicon: chromosome
• Accession: NZ_CP074448
• Organism: Enterobacter asburiae strain S3_CRE3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KHV90_RS21080
• Protein ID: WP_080298642.1
• Coordinates: 4431627..4431998 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A808IJD0
• Locus tag: KHV90_RS21085
• Protein ID: WP_023309753.1
• Coordinates: 4432001..4432243 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHV90_RS21065 (KHV90_21015)	4429127..4430029	+	903	WP_008501831.1	formate dehydrogenase subunit beta	-
KHV90_RS21070 (KHV90_21020)	4430026..4430661	+	636	WP_010436852.1	formate dehydrogenase cytochrome b556 subunit	-
KHV90_RS21075 (KHV90_21025)	4430658..4431587	+	930	WP_029741588.1	formate dehydrogenase accessory protein FdhE	-
KHV90_RS21080 (KHV90_21030)	4431627..4431998	-	372	WP_080298642.1	type II toxin-antitoxin system VapC family toxin	Toxin
KHV90_RS21085 (KHV90_21035)	4432001..4432243	-	243	WP_023309753.1	ribbon-helix-helix protein, CopG family	Antitoxin
KHV90_RS21090 (KHV90_21040)	4432443..4433363	+	921	WP_048981029.1	alpha/beta hydrolase	-
KHV90_RS21095 (KHV90_21045)	4433372..4434313	-	942	WP_039264191.1	fatty acid biosynthesis protein FabY	-
KHV90_RS21100 (KHV90_21050)	4434358..4434795	-	438	WP_008501836.1	D-aminoacyl-tRNA deacylase	-
KHV90_RS21105 (KHV90_21055)	4434792..4435673	-	882	WP_033146965.1	virulence factor BrkB family protein	-
KHV90_RS21110 (KHV90_21060)	4435667..4436266	-	600	WP_039264189.1	glucose-1-phosphatase	-
KHV90_RS21115 (KHV90_21065)	4436355..4436825	-	471	WP_033146967.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-109)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13688.85 Da
• Isoelectric Point: 6.9789

>T202201 WP_080298642.1 NZ_CP074448:c4431998-4431627 [Enterobacter asburiae]
MEHMAVFDTNILIDLFNNRVEAADAIDRTASHRAISLITWMEVMVGARKHGHEAKTATVMGVFEIIDITRDIAERSVILR
EKHGMKLPDAIILATAQSRNCPLISRNTKDFSGITGVVSPYQL

Nucleotide

• Length: 372 bp

>T202201 NZ_CP099344:336615-336718 [Escherichia marmotae]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9411.53 Da
• Isoelectric Point: 4.2803

>AT202201 WP_023309753.1 NZ_CP074448:c4432243-4432001 [Enterobacter asburiae]
ISLSADLDMGRILIDLSDDVIQRLDNLKQLRNRPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT202201 NZ_CP099344:c336732-336487 [Escherichia marmotae]
CTGGCTTTATAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCG
CTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTCCGCGCG
TAAAGTGGTCAGAAAAAAAGCGCGTTTATCATCCTCTAAAATGTTAAAAACCGCCCGTTCTGGTGAAAGCACCCGGGCGG
TTTTTT

Structures

Antitoxin

• AlphaFold DB: A0A808IJD0