Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3134767..3135343 Replicon chromosome
Accession NZ_CP074448
Organism Enterobacter asburiae strain S3_CRE3

Toxin (Protein)


Gene name shpA Uniprot ID A0A808IJQ6
Locus tag KHV90_RS14830 Protein ID WP_048980720.1
Coordinates 3135056..3135343 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A808I9W7
Locus tag KHV90_RS14825 Protein ID WP_048980603.1
Coordinates 3134767..3135069 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV90_RS14800 (KHV90_14760) 3130874..3131419 + 546 WP_023310269.1 YfaZ family outer membrane protein -
KHV90_RS14805 (KHV90_14765) 3131419..3131739 + 321 WP_039262264.1 hypothetical protein -
KHV90_RS14810 (KHV90_14770) 3131785..3133155 - 1371 WP_048980598.1 NAD-dependent succinate-semialdehyde dehydrogenase -
KHV90_RS14815 (KHV90_14775) 3133307..3134050 + 744 WP_048980600.1 AraC family transcriptional regulator -
KHV90_RS14820 (KHV90_14780) 3134098..3134736 + 639 WP_048980602.1 LysE family translocator -
KHV90_RS14825 (KHV90_14785) 3134767..3135069 - 303 WP_048980603.1 BrnA antitoxin family protein Antitoxin
KHV90_RS14830 (KHV90_14790) 3135056..3135343 - 288 WP_048980720.1 BrnT family toxin Toxin
KHV90_RS14835 (KHV90_14795) 3135514..3136785 + 1272 WP_048980604.1 DUF445 domain-containing protein -
KHV90_RS14840 (KHV90_14800) 3136782..3137858 - 1077 WP_023310256.1 DUF2955 domain-containing protein -
KHV90_RS14845 (KHV90_14805) 3137863..3138930 - 1068 WP_101744430.1 HlyD family secretion protein -
KHV90_RS14850 (KHV90_14810) 3138927..3139397 - 471 WP_033144545.1 MarR family transcriptional regulator -
KHV90_RS14855 (KHV90_14815) 3139547..3140242 - 696 WP_048980606.1 winged helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(32-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11201.66 Da        Isoelectric Point: 6.7609

>T202198 WP_048980720.1 NZ_CP074448:c3135343-3135056 [Enterobacter asburiae]
MPTEFEWDTNKAKSNLIKHGIRFEEAVLVFDDPYHLSLQDRHENGEFRWQTIGLVHGLIVIMVAHTVRFESGDEVIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T202198 NZ_CP099341:2053-2328 [Escherichia fergusonii]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTACTGGCCAGTAAACCTGC
TGCTGCCAGAACGGGGCAGTCCCTGACACAGGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACGCACGAGAAAACAGGTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11451.16 Da        Isoelectric Point: 10.1522

>AT202198 WP_048980603.1 NZ_CP074448:c3135069-3134767 [Enterobacter asburiae]
MSMVKYKRGSAPEMSARREAELKALAEKSEAEIDYSDIPAMGDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT202198 NZ_CP099341:1769-2053 [Escherichia fergusonii]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCCATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCTTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A808IJQ6

Antitoxin

Source ID Structure
AlphaFold DB A0A808I9W7

References