Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2676744..2677364 Replicon chromosome
Accession NZ_CP074448
Organism Enterobacter asburiae strain S3_CRE3

Toxin (Protein)


Gene name Hha Uniprot ID A0A1H8SI38
Locus tag KHV90_RS12675 Protein ID WP_008499287.1
Coordinates 2677146..2677364 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag KHV90_RS12670 Protein ID WP_008499288.1
Coordinates 2676744..2677118 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV90_RS12660 (KHV90_12620) 2671870..2673063 + 1194 WP_029741963.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KHV90_RS12665 (KHV90_12625) 2673086..2676232 + 3147 WP_029741964.1 multidrug efflux RND transporter permease subunit AcrB -
KHV90_RS12670 (KHV90_12630) 2676744..2677118 + 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
KHV90_RS12675 (KHV90_12635) 2677146..2677364 + 219 WP_008499287.1 HHA domain-containing protein Toxin
KHV90_RS12680 (KHV90_12640) 2677570..2678121 + 552 WP_023310599.1 maltose O-acetyltransferase -
KHV90_RS12685 (KHV90_12645) 2678240..2678707 + 468 WP_023310598.1 YlaC family protein -
KHV90_RS12690 (KHV90_12650) 2678679..2680139 - 1461 WP_048978928.1 PLP-dependent aminotransferase family protein -
KHV90_RS12695 (KHV90_12655) 2680241..2680951 + 711 WP_048978929.1 GNAT family protein -
KHV90_RS12700 (KHV90_12660) 2680948..2681088 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
KHV90_RS12705 (KHV90_12665) 2681091..2681351 - 261 WP_010428154.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T202197 WP_008499287.1 NZ_CP074448:2677146-2677364 [Enterobacter asburiae]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T202197 NZ_CP099340:c44512-44132 [Escherichia fergusonii]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
TTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT202197 WP_008499288.1 NZ_CP074448:2676744-2677118 [Enterobacter asburiae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT202197 NZ_CP099340:c44733-44512 [Escherichia fergusonii]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8SI38

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References