Detailed information of TA system TA202181

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2609685..2610424
• Replicon: chromosome
• Accession: NZ_CP074446
• Organism: Klebsiella aerogenes strain S2_CRE2

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: KHV89_RS12250
• Protein ID: WP_103825819.1
• Coordinates: 2609939..2610424 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A0H3FSM9
• Locus tag: KHV89_RS12245
• Protein ID: WP_015705366.1
• Coordinates: 2609685..2609951 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHV89_RS12225 (2605679)	2605679..2606923	+	1245	WP_270097723.1	ATP-binding protein	-
KHV89_RS12230 (2606923)	2606923..2607540	+	618	WP_032711621.1	response regulator	-
KHV89_RS12235 (2607636)	2607636..2608133	+	498	WP_103825818.1	heme-binding protein	-
KHV89_RS12240 (2608177)	2608177..2609412	-	1236	WP_020078885.1	MFS transporter	-
KHV89_RS12245 (2609685)	2609685..2609951	+	267	WP_015705366.1	DUF1778 domain-containing protein	Antitoxin
KHV89_RS12250 (2609939)	2609939..2610424	+	486	WP_103825819.1	GNAT family N-acetyltransferase	Toxin
KHV89_RS12255 (2610496)	2610496..2612112	+	1617	WP_032711617.1	FAD-NAD(P)-binding protein	-
KHV89_RS12260 (2612231)	2612231..2613331	+	1101	WP_015705363.1	NADH:flavin oxidoreductase/NADH oxidase	-
KHV89_RS12265 (2613388)	2613388..2613546	-	159	WP_002903230.1	YqaE/Pmp3 family membrane protein	-
KHV89_RS12270 (2613807)	2613807..2614877	+	1071	WP_103825820.1	mannonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (25-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17577.23 Da
• Isoelectric Point: 8.8793

>T202181 WP_103825819.1 NZ_CP074446:2609939-2610424 [Klebsiella aerogenes]
MGKISAPAPLSSHHQIAEFCCGETVLDQWLKQRGMKNQAQGAARTFVVCKEDSHQVVGFYSLATGSVNHSEATGGLRRNM
PDPIPVIILARLAIDCAFHGQGLGADLLHDALLRSYRVAENVGVRALMVHALTDSAKRFYLHHGFKASTTQERTLFLALP
K

Nucleotide

• Length: 486 bp

>T202181 NZ_CP099338:748502-748816 [Escherichia fergusonii]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCATATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACACGCTGGGA
ACGAAATTTTGCGATGTCAGCGCGCACCGAACTTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10085.56 Da
• Isoelectric Point: 4.6558

>AT202181 WP_015705366.1 NZ_CP074446:2609685-2609951 [Klebsiella aerogenes]
MKSDIQLNIRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNLSDEQYAEFIDMLDAPVEANPALDKLL
ARKPQWEK

Nucleotide

• Length: 267 bp

>AT202181 NZ_CP099338:748266-748499 [Escherichia fergusonii]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3FSM9