Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 15337..15980 Replicon plasmid pCRE1_2
Accession NZ_CP074444
Organism Klebsiella pneumoniae strain S1_CRE1

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag KHV88_RS27695 Protein ID WP_000754566.1
Coordinates 15564..15980 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag KHV88_RS27690 Protein ID WP_001261276.1
Coordinates 15337..15567 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV88_RS27670 12609..12788 + 180 Protein_14 hypothetical protein -
KHV88_RS27675 (KHV88_27600) 12846..13622 - 777 WP_000015958.1 site-specific integrase -
KHV88_RS27680 (KHV88_27605) 13619..14362 - 744 WP_000129823.1 hypothetical protein -
KHV88_RS27685 (KHV88_27610) 14413..14763 - 351 WP_000493378.1 hypothetical protein -
KHV88_RS27690 (KHV88_27615) 15337..15567 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KHV88_RS27695 (KHV88_27620) 15564..15980 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
KHV88_RS27700 (KHV88_27625) 16184..17040 + 857 Protein_20 IS3-like element ISEc15 family transposase -
KHV88_RS27705 (KHV88_27630) 17322..18398 + 1077 WP_000227969.1 IS110 family transposase -
KHV88_RS27710 (KHV88_27635) 19039..19122 + 84 Protein_22 SOS response-associated peptidase -
KHV88_RS27715 (KHV88_27640) 19285..20166 - 882 Protein_23 Tn3 family transposase -
KHV88_RS27720 (KHV88_27645) 20228..20980 + 753 WP_000602738.1 IS6 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aac(6')-Ib / aph(4)-Ia / aac(3)-IVa / sul3 / ant(3'')-Ia / cmlA1 / aadA2 - 1..45175 45175
- inside IScluster/Tn aac(6')-Ib / aph(4)-Ia / aac(3)-IVa / sul3 / ant(3'')-Ia / cmlA1 / aadA2 - 7007..43230 36223


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T202170 WP_000754566.1 NZ_CP074444:15564-15980 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T202170 NZ_CP099331:c4195034-4194927 [Escherichia fergusonii]
ATGACGCTCGCTGAGTTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATCGCTGGCATTCTTGCCAGTTTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT202170 WP_001261276.1 NZ_CP074444:15337-15567 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT202170 NZ_CP099331:4195082-4195148 [Escherichia fergusonii]
GGTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References