Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5442692..5443317 Replicon chromosome
Accession NZ_CP074442
Organism Klebsiella pneumoniae strain S1_CRE1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KHV88_RS26950 Protein ID WP_002882817.1
Coordinates 5442692..5443075 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KHV88_RS26955 Protein ID WP_004150355.1
Coordinates 5443075..5443317 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV88_RS26935 (KHV88_26865) 5440058..5440960 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KHV88_RS26940 (KHV88_26870) 5440957..5441592 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KHV88_RS26945 (KHV88_26875) 5441589..5442518 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KHV88_RS26950 (KHV88_26880) 5442692..5443075 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KHV88_RS26955 (KHV88_26885) 5443075..5443317 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KHV88_RS26960 (KHV88_26890) 5443522..5444439 + 918 WP_002882812.1 alpha/beta hydrolase -
KHV88_RS26965 (KHV88_26895) 5444453..5445394 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KHV88_RS26970 (KHV88_26900) 5445439..5445876 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KHV88_RS26975 (KHV88_26905) 5445873..5446733 - 861 WP_002882807.1 virulence factor BrkB family protein -
KHV88_RS26980 (KHV88_26910) 5446727..5447326 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T202169 WP_002882817.1 NZ_CP074442:c5443075-5442692 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T202169 NZ_CP099331:c4194069-4193962 [Escherichia fergusonii]
ATGACGCTCGCTGAGTTGGGCATAGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTACTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT202169 WP_004150355.1 NZ_CP074442:c5443317-5443075 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT202169 NZ_CP099331:4194127-4194181 [Escherichia fergusonii]
CAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References