Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4855034..4855844 Replicon chromosome
Accession NZ_CP074442
Organism Klebsiella pneumoniae strain S1_CRE1

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag KHV88_RS24130 Protein ID WP_002887280.1
Coordinates 4855034..4855567 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KHV88_RS24135 Protein ID WP_002887278.1
Coordinates 4855578..4855844 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV88_RS24125 (KHV88_24065) 4853865..4854986 + 1122 WP_002887282.1 cupin domain-containing protein -
KHV88_RS24130 (KHV88_24070) 4855034..4855567 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
KHV88_RS24135 (KHV88_24075) 4855578..4855844 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KHV88_RS24140 (KHV88_24080) 4855947..4857380 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
KHV88_RS24145 (KHV88_24085) 4857370..4858053 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KHV88_RS24150 (KHV88_24090) 4858225..4859610 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
KHV88_RS24155 (KHV88_24095) 4859628..4859972 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T202166 WP_002887280.1 NZ_CP074442:c4855567-4855034 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T202166 NZ_CP099331:3267932-3268150 [Escherichia fergusonii]
ATGTCCGATAAGCCTTTAACCAAAACTGATTATTTGATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAACGTGT
CATCGAAAAAAATAAATACGAACTCTCTGACAATGAACTGGCGGTATTTTATTCAGCAGCTGATCACCGTCTCGCTGAAT
TGACCATGAATAAACTTTACGACAAGATCCCCTCCTCCGTATGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT202166 WP_002887278.1 NZ_CP074442:c4855844-4855578 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT202166 NZ_CP099331:3267531-3267905 [Escherichia fergusonii]
ATGGACGAATACTCACCAAAACGACATGATATCGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAATCTTGAAGAGAGCAACCATGGCTGGGTTAACGACCCAACCTCGGCTGTCAATCTGCAGCTCAACGAACTGATTGAGC
ATATTGCAACCTTCGCACTTAATTACAAAATTAAGTATAATGAGGACAATAAGCTCATAGAGCAGATCGATGAATATCTG
GATGACACTTTTATGTTGTTCAGTAGTTATGGCATTAATACACAGGATTTACAGAAATGGCGAAAATCTGGAAACAGGCT
CTTCCGCTGTTTTGTTAACGCCACCAGGGCGAACCCTGTTAGTTTGTCGTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References