Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4242343..4242962 Replicon chromosome
Accession NZ_CP074442
Organism Klebsiella pneumoniae strain S1_CRE1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KHV88_RS21185 Protein ID WP_002892050.1
Coordinates 4242744..4242962 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KHV88_RS21180 Protein ID WP_002892066.1
Coordinates 4242343..4242717 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV88_RS21170 (KHV88_21110) 4237495..4238688 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KHV88_RS21175 (KHV88_21115) 4238711..4241857 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KHV88_RS21180 (KHV88_21120) 4242343..4242717 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KHV88_RS21185 (KHV88_21125) 4242744..4242962 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KHV88_RS21190 (KHV88_21130) 4243121..4243687 + 567 WP_002892030.1 maltose O-acetyltransferase -
KHV88_RS21195 (KHV88_21135) 4243659..4243799 - 141 WP_004147370.1 hypothetical protein -
KHV88_RS21200 (KHV88_21140) 4243820..4244290 + 471 WP_002892026.1 YlaC family protein -
KHV88_RS21205 (KHV88_21145) 4244265..4245716 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KHV88_RS21210 (KHV88_21150) 4245817..4246515 + 699 WP_002892021.1 GNAT family protein -
KHV88_RS21215 (KHV88_21155) 4246512..4246652 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KHV88_RS21220 (KHV88_21160) 4246652..4246915 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T202165 WP_002892050.1 NZ_CP074442:4242744-4242962 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T202165 NZ_CP099331:c2755703-2755452 [Escherichia fergusonii]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAACGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCCCTGGAAGAGACTGCTTATC
TATTGCGTTCCCCCGCTAATGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT202165 WP_002892066.1 NZ_CP074442:4242343-4242717 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT202165 NZ_CP099331:c2755455-2755201 [Escherichia fergusonii]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACTCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References