Detailed information of TA system TA202155

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1052241..1052977
• Replicon: chromosome
• Accession: NZ_CP074442
• Organism: Klebsiella pneumoniae strain S1_CRE1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: KHV88_RS05325
• Protein ID: WP_003026803.1
• Coordinates: 1052495..1052977 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: KHV88_RS05320
• Protein ID: WP_003026799.1
• Coordinates: 1052241..1052507 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHV88_RS05275 (KHV88_05265)	1048303..1048665	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
KHV88_RS05280 (KHV88_05270)	1048715..1049065	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
KHV88_RS05285 (KHV88_05275)	1049423..1049692	+	270	WP_004152102.1	hypothetical protein	-
KHV88_RS05290 (KHV88_05280)	1049680..1050255	+	576	WP_004152103.1	hypothetical protein	-
KHV88_RS05295 (KHV88_05285)	1050286..1050780	+	495	WP_004152104.1	DNA-binding protein	-
KHV88_RS05300 (KHV88_05290)	1050824..1051192	+	369	WP_004152105.1	hypothetical protein	-
KHV88_RS05305 (KHV88_05295)	1051226..1051429	+	204	WP_004152106.1	HHA domain-containing protein	-
KHV88_RS05310 (KHV88_05300)	1051478..1051735	+	258	WP_004152107.1	hypothetical protein	-
KHV88_RS05315 (KHV88_05305)	1051811..1052065	+	255	WP_004152108.1	hypothetical protein	-
KHV88_RS05320 (KHV88_05310)	1052241..1052507	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
KHV88_RS05325 (KHV88_05315)	1052495..1052977	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
KHV88_RS05330 (KHV88_05320)	1053189..1054535	+	1347	WP_020314316.1	ISNCY family transposase	-
KHV88_RS05335	1054696..1054827	+	132	WP_004218042.1	hypothetical protein	-
KHV88_RS05340 (KHV88_05325)	1055036..1056174	-	1139	Protein_1052	IS3 family transposase	-
KHV88_RS05345 (KHV88_05330)	1056378..1057340	-	963	WP_004152113.1	zinc metalloprotease HtpX	-
KHV88_RS05350 (KHV88_05335)	1057327..1057815	-	489	WP_004152114.1	phosphate-starvation-inducible PsiE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / catA1	-	1051226..1096660	45434

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T202155 WP_003026803.1 NZ_CP074442:1052495-1052977 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T202155 NZ_CP099328:c4355794-4355642 [Escherichia fergusonii]
ATGCCGCAGAAATACGGATTACTTTCGTTAATAGTGATTTGTTTCACGCTTTTATTTTTCACCTGGATGATAAGAGATTC
ACTGTGTGAATTGCATATTAAACAGGAGAGTTATGAGCTGGCGGCGTTTTTAGCCTGCAAATTGAAAGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT202155 WP_003026799.1 NZ_CP074442:1052241-1052507 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT202155 NZ_CP099328:4355851-4355899 [Escherichia fergusonii]
GCATAGAGGGGCCTCACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTG

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66