Detailed information of TA system TA202150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3920149..3920888
• Replicon: chromosome
• Accession: NZ_CP074437
• Organism: Klebsiella sp. A52

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: KGA69_RS18480
• Protein ID: WP_045394429.1
• Coordinates: 3920403..3920888 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A0H3FSM9
• Locus tag: KGA69_RS18475
• Protein ID: WP_015705366.1
• Coordinates: 3920149..3920415 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGA69_RS18450 (3915482)	3915482..3916069	+	588	WP_045394417.1	hypothetical protein	-
KGA69_RS18455 (3916066)	3916066..3917385	+	1320	WP_049060916.1	ATP-binding protein	-
KGA69_RS18460 (3917385)	3917385..3918002	+	618	WP_045394421.1	response regulator	-
KGA69_RS18465 (3918098)	3918098..3918595	+	498	WP_049061207.1	heme-binding protein	-
KGA69_RS18470 (3918641)	3918641..3919876	-	1236	WP_047067471.1	MFS transporter	-
KGA69_RS18475 (3920149)	3920149..3920415	+	267	WP_015705366.1	DUF1778 domain-containing protein	Antitoxin
KGA69_RS18480 (3920403)	3920403..3920888	+	486	WP_045394429.1	GNAT family N-acetyltransferase	Toxin
KGA69_RS18485 (3920960)	3920960..3922573	+	1614	WP_213292558.1	FAD-NAD(P)-binding protein	-
KGA69_RS18490 (3922692)	3922692..3923792	+	1101	WP_213292559.1	NADH:flavin oxidoreductase/NADH oxidase	-
KGA69_RS18495 (3923847)	3923847..3924005	-	159	WP_002903230.1	YqaE/Pmp3 family membrane protein	-
KGA69_RS18500 (3924266)	3924266..3925336	+	1071	WP_045394436.1	mannonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17575.20 Da
• Isoelectric Point: 8.8793

>T202150 WP_045394429.1 NZ_CP074437:3920403-3920888 [Klebsiella sp. A52]
MGKVSAPTPLSSHHQIAEFCCGETVLDQWLKQRGLKNQAQGAARTFVVCKEDSHQVVGFYSLATGSVNHTEATGGLRRNM
PDPIPVIILARLAIDCAFHGQGLGGDLLHDALLRSYRVAENVGVRALMVHALTDSAKRFYLHHGFKASTTQERTLFLALP
K

Nucleotide

• Length: 486 bp

>T202150 NZ_CP099328:c3858410-3858123 [Escherichia fergusonii]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGATGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10085.56 Da
• Isoelectric Point: 4.6558

>AT202150 WP_015705366.1 NZ_CP074437:3920149-3920415 [Klebsiella sp. A52]
MKSDIQLNIRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNLSDEQYAEFIDMLDAPVEANPALDKLL
ARKPQWEK

Nucleotide

• Length: 267 bp

>AT202150 NZ_CP099328:c3858136-3857834 [Escherichia fergusonii]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAATAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3FSM9