Detailed information of TA system TA202139

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1439733..1440754
• Replicon: chromosome
• Accession: NZ_CP074437
• Organism: Klebsiella sp. A52

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: A0A0G9UXE3
• Locus tag: KGA69_RS06700
• Protein ID: WP_032706547.1
• Coordinates: 1440179..1440754 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: KGA69_RS06695
• Protein ID: WP_213292926.1
• Coordinates: 1439733..1440179 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGA69_RS06670 (1435254)	1435254..1436033	+	780	WP_045390545.1	MBL fold metallo-hydrolase	-
KGA69_RS06675 (1436055)	1436055..1437008	+	954	WP_045390821.1	helix-turn-helix domain-containing protein	-
KGA69_RS06680 (1437005)	1437005..1438054	-	1050	WP_045390547.1	NAD(P)-dependent alcohol dehydrogenase	-
KGA69_RS06685 (1438212)	1438212..1438514	-	303	WP_045390549.1	putative quinol monooxygenase	-
KGA69_RS06690 (1438515)	1438515..1439522	-	1008	WP_045390550.1	zinc-binding alcohol dehydrogenase family protein	-
KGA69_RS06695 (1439733)	1439733..1440179	+	447	WP_213292926.1	helix-turn-helix domain-containing protein	Antitoxin
KGA69_RS06700 (1440179)	1440179..1440754	+	576	WP_032706547.1	PIN domain-containing protein	Toxin
KGA69_RS06705 (1441060)	1441060..1442256	+	1197	WP_015703847.1	hypothetical protein	-
KGA69_RS06710 (1442361)	1442361..1442648	-	288	Protein_1295	SymE family type I addiction module toxin	-
KGA69_RS06720 (1443480)	1443480..1444874	+	1395	WP_045390552.1	glycoside-pentoside-hexuronide family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• SinI-like, DNA-binding domain (79-126)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21688.29 Da
• Isoelectric Point: 6.2900

>T202139 WP_032706547.1 NZ_CP074437:1440179-1440754 [Klebsiella sp. A52]
MRHSPYPVILDACVLYPARLRDLLMHLGIAGLYQPKWSRFIHDEWCRNLLINRPDIAPEALRRTVDLMNAALPDANVTGF
AGLINGLVLPDPDDRHVLAAAIRAKAEIIVTLNHKDFPAESLLPFEIETLHPDVFISDLFDLNHALALEAVRRQRQSLKH
PPMTVDEFLEMLLKQGLPMTVKELANYQYAI

Nucleotide

• Length: 576 bp

>T202139 NZ_CP099327:c2848914-2848811 [Enterobacter ludwigii]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16714.23 Da
• Isoelectric Point: 6.2257

>AT202139 WP_213292926.1 NZ_CP074437:1439733-1440179 [Klebsiella sp. A52]
MTKLNLPDPQESELAQRGQRELAAYLSTHLETQRIAIVGEDDKTHTIELPTSALTMLMEILGELACGNAVQIVPVHAELT
TQEAANILNVSRPHMVKLLEARKLPFHKTGRHRRVLFADLMEYKKRREQESLDAMQALADQAQDLGMY

Nucleotide

• Length: 447 bp

>AT202139 NZ_CP099327:2848797-2848949 [Enterobacter ludwigii]
GCTTTCAATCAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCG
CTAAAAGTTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGT

Structures

Toxin

• AlphaFold DB: A0A0G9UXE3