Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 73903..74522 Replicon chromosome
Accession NZ_CP074437
Organism Klebsiella sp. A52

Toxin (Protein)


Gene name Hha Uniprot ID A0A0H3FXE2
Locus tag KGA69_RS00335 Protein ID WP_015367918.1
Coordinates 74304..74522 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A837JZD4
Locus tag KGA69_RS00330 Protein ID WP_045393536.1
Coordinates 73903..74277 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGA69_RS00320 (69062) 69062..70261 + 1200 WP_045393531.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KGA69_RS00325 (70284) 70284..73430 + 3147 WP_045393534.1 multidrug efflux RND transporter permease subunit AcrB -
KGA69_RS00330 (73903) 73903..74277 + 375 WP_045393536.1 Hha toxicity modulator TomB Antitoxin
KGA69_RS00335 (74304) 74304..74522 + 219 WP_015367918.1 HHA domain-containing protein Toxin
KGA69_RS00340 (74654) 74654..75214 + 561 WP_045393539.1 maltose O-acetyltransferase -
KGA69_RS00345 (75343) 75343..75807 + 465 WP_047047056.1 YlaC family protein -
KGA69_RS00350 (75782) 75782..77236 - 1455 WP_213293123.1 PLP-dependent aminotransferase family protein -
KGA69_RS00355 (77339) 77339..78049 + 711 WP_045393548.1 GNAT family protein -
KGA69_RS00360 (78046) 78046..78186 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
KGA69_RS00365 (78189) 78189..78449 - 261 WP_015367923.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8569.96 Da        Isoelectric Point: 9.4828

>T202137 WP_015367918.1 NZ_CP074437:74304-74522 [Klebsiella sp. A52]
MSGKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T202137 NZ_CP099327:478138-478422 [Enterobacter ludwigii]
ATGAGTTATGAACTCGTATTTGATCCGAGGGCGTTCAAGGAGTGGACCAGGCTGGGTGATACCGTAAAAAATCAGTTTAA
GAAGAAGCTTGCGAACGTGTTGGTGAACCCCAGGGTTGAATCGGCTCGCCTGTATGGCCTGCCTGATTGCGACAAGATAA
AACTCAGATCCCAGGGTTATCGTTTGGTCTATCAGGTTCATGACAACGTTGTGACGGTATGTGTTATTGCTATCGGTAAG
AGAGAGAAATCAGCCGTTTCTCATGACGTAAACAAACGGCTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14492.26 Da        Isoelectric Point: 4.9045

>AT202137 WP_045393536.1 NZ_CP074437:73903-74277 [Klebsiella sp. A52]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNRGWVNDPTSAVNLQLNELIEHIATYALNYKIKYAEDNKLISQLDEYL
DDTFMLFSSYGINTSDLQKWRKSGNRLFRCFVNASRENPVSLSC

Download         Length: 375 bp

>AT202137 NZ_CP099327:477906-478148 [Enterobacter ludwigii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTTGAAAAACTCAATATCACACC
AACGGAAGCCGTTCGGTTACTGTTTCAGTACGTTGCGGAGACGGGGCGTATGCCGGTGAAAACGGTCACTGTTAGCGACA
GTGAAGATGAACTCATACAGACAGTCCGGGAGCGGCTGGCTAATCCGCAAAAAGGAATTAAGGTTAGGCTGGATGAGTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3FXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A837JZD4

References