Detailed information of TA system TA202136

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 60947..61472
• Replicon: plasmid p14523B
• Accession: NZ_CP074430
• Organism: Salmonella sp. SJTUF14523

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: I3W3D5
• Locus tag: KFD69_RS23515
• Protein ID: WP_001159863.1
• Coordinates: 61167..61472 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7S5D0
• Locus tag: KFD69_RS23510
• Protein ID: WP_000813641.1
• Coordinates: 60947..61165 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFD69_RS23480 (KFD69_23465)	56561..56947	+	387	WP_000751876.1	YgiW/YdeI family stress tolerance OB fold protein	-
KFD69_RS23485 (KFD69_23470)	57000..57119	+	120	Protein_70	recombinase	-
KFD69_RS23490 (KFD69_23475)	57488..58477	-	990	WP_000461382.1	RepB family plasmid replication initiator protein	-
KFD69_RS23495 (KFD69_23480)	58971..59266	-	296	Protein_72	cytoplasmic protein	-
KFD69_RS23500 (KFD69_23485)	59278..59706	+	429	Protein_73	hypothetical protein	-
KFD69_RS23505 (KFD69_23490)	59750..60271	-	522	WP_077681952.1	hypothetical protein	-
KFD69_RS23510 (KFD69_23495)	60947..61165	+	219	WP_000813641.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
KFD69_RS23515 (KFD69_23500)	61167..61472	+	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	Toxin
KFD69_RS23520 (KFD69_23505)	61474..61764	+	291	WP_001266176.1	hypothetical protein	-
KFD69_RS23525 (KFD69_23510)	61761..62282	+	522	WP_000198608.1	hypothetical protein	-
KFD69_RS23530 (KFD69_23515)	62317..63099	+	783	WP_000082169.1	site-specific integrase	-
KFD69_RS23535 (KFD69_23520)	63108..63821	+	714	WP_000545756.1	EAL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B	fdeC / spvC / spvB / rck / pefD / pefC / pefA / pefB	1..64327	64327

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11587.48 Da
• Isoelectric Point: 6.9787

>T202136 WP_001159863.1 NZ_CP074430:61167-61472 [Salmonella sp. SJTUF14523]
MQFKVYTCKRESRYRLFVDVQSDIIDTPGRRMAVPLVSARLLSEKVPRDLYPVMHIGDEPYRLLTTDMTSVPATVIGEEV
ADLSLRENDIKNAINLMFRGI

Nucleotide

• Length: 306 bp

>T202136 NZ_CP099326:c41600-41115 [Enterobacter ludwigii]
GTGGGACAAGTAACTGCACCAGAACCGTTATCCAGCTCCCATCAACTTGCTGAATTCGTCAGTGGTGAGGCTGTACTTGA
TGACTGGCTTAAACAGAGAGGATTAAAAAATCAGGCTCTCGGTTCAACCCGAACGTTTGTTGTCTGTAAAACATACACGA
AGCAGGTTGTCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCATGTGGAAGCCACGGGAAGTCTCAGGCGCAATATG
CCTGATCCTATTCCTGTTATCATTCTTGCCCGTCTGGCTGTTGATGCTTCATTCCGGGGAAAAGGGCTAGGTGCTGATTT
ACTGCACGACGCAGTATTGCGCTGTTACCGGGTCGCAGAAAATATAGGCGTACGCGCGATTATGGTTCATGCACTGACTG
AAGAGGCTAAAAGCTTTTATCTCCACCATGGTTTTAAGGCGTCGCAAACCCAGGAAAGGACTCTGTTCCTCAAACTCCCA
CAGTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8314.16 Da
• Isoelectric Point: 4.5203

>AT202136 WP_000813641.1 NZ_CP074430:60947-61165 [Salmonella sp. SJTUF14523]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADDNRNW

Nucleotide

• Length: 219 bp

>AT202136 NZ_CP099326:c41854-41588 [Enterobacter ludwigii]
ATGAAATCAGATGTTCAGCTCAATCTCAGAGCCAAGGAGTCTCAAAGAGCACTTATCGATGCTGCTGCAGAGATCCTCCA
TAAATCACGCACAGACTTTATCCTTGAGATGGCCTGCCAGGCTGCGGAGAATGTGATCCTCGATCGTCGTGTATTCAATT
TTAACGATGAGCAGTATGCAGAGTTTATCGAGATGCTTGATGCACCAGTTTCGGATGATGCTGCCGTTGATAAACTGCTG
GCAAGGAAACCGCAGTGGGACAAGTAA

Structures

Toxin

• AlphaFold DB: I3W3D5

Antitoxin

• AlphaFold DB: A0A656ICA6