Detailed information of TA system TA202070

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 26386..26954
• Replicon: plasmid unnamed2
• Accession: NZ_CP074418
• Organism: Vibrio parahaemolyticus strain 64

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1Y1BA95
• Locus tag: J5O00_RS25160
• Protein ID: WP_023624650.1
• Coordinates: 26613..26954 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: J5O00_RS25155
• Protein ID: WP_122016154.1
• Coordinates: 26386..26616 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5O00_RS25120	21803..22435	-	633	WP_257576302.1	Ig-like domain-containing protein	-
J5O00_RS25125	22537..22752	-	216	WP_219079452.1	Ig-like domain-containing protein	-
J5O00_RS25130	23315..23689	-	375	WP_127914005.1	hypothetical protein	-
J5O00_RS25135	23999..24220	+	222	WP_023624649.1	hypothetical protein	-
J5O00_RS26830	24840..24986	+	147	WP_257576293.1	recombinase family protein	-
J5O00_RS26835	24988..25128	+	141	WP_257576303.1	recombinase family protein	-
J5O00_RS25145	25310..25735	-	426	Protein_33	site-specific integrase	-
J5O00_RS25150	25779..25934	-	156	WP_219079453.1	hypothetical protein	-
J5O00_RS25155	26386..26616	+	231	WP_122016154.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
J5O00_RS25160	26613..26954	+	342	WP_023624650.1	endoribonuclease MazF	Toxin
J5O00_RS25165	27337..27834	-	498	WP_257576294.1	hypothetical protein	-
J5O00_RS25170	27834..28565	-	732	WP_023624652.1	AHH domain-containing protein	-
J5O00_RS26840	28858..28919	-	62	Protein_39	DUF3265 domain-containing protein	-
J5O00_RS26845	29810..30160	-	351	WP_257576295.1	hypothetical protein	-
J5O00_RS26850	30102..30671	-	570	WP_257576296.1	replication initiation protein	-
J5O00_RS26855	30706..30885	-	180	WP_257576297.1	hypothetical protein	-
J5O00_RS25180	31219..31859	-	641	Protein_43	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..109074	109074
	-	flank	IS/Tn	-	-	31219..31359	140

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-107)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12159.65 Da
• Isoelectric Point: 5.8124

>T202070 WP_023624650.1 NZ_CP074418:26613-26954 [Vibrio parahaemolyticus]
MTYAVPEKGDVVVMSFNPQSGHEQAGDSRPGLVISPKEFNQATGFALVCPITNQSKGYPFEVEITGSQKTTGVVLADQVK
SLDWRARRARVVDSVSNETLGTVRDYVTAIANG

Nucleotide

• Length: 342 bp

>T202070 NZ_CP099306:4537021-4537239 [Klebsiella michiganensis]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCAGCGGCCGACCATCGTCTTGCTGAGC
TGACGATGAATAAACTGTATGACAAGATCCCCCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8499.87 Da
• Isoelectric Point: 6.4604

>AT202070 WP_122016154.1 NZ_CP074418:26386-26616 [Vibrio parahaemolyticus]
MSQTIAKWGHSAAVRIPSKVLKNMDMAVGDKVEVKVFNRQIIIEPAKPTLAQILSQITDENRHDEVITETMGNELL

Nucleotide

• Length: 231 bp

>AT202070 NZ_CP099306:4536620-4536994 [Klebsiella michiganensis]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGACCCGACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCACTTAATTATAAAATTAAGTACGCAGAGGATAATAAGCTGATTACCCAGATTGATGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGCGCAGATCTGCAGAAATGGCGTAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCTGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A1Y1BA95