Detailed information of TA system TA202063

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 5717315..5717913
• Replicon: chromosome
• Accession: NZ_CP074412
• Organism: Pseudomonas viridiflava strain U625

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: KHW14_RS25175
• Protein ID: WP_069145198.1
• Coordinates: 5717315..5717662 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: -
• Locus tag: KHW14_RS25180
• Protein ID: WP_122409396.1
• Coordinates: 5717659..5717913 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHW14_RS25160 (KHW14_25160)	5712766..5714763	-	1998	WP_122770408.1	transketolase	-
KHW14_RS25165 (KHW14_25165)	5715022..5716017	+	996	WP_043305547.1	metalloregulator ArsR/SmtB family transcription factor	-
KHW14_RS25170 (KHW14_25170)	5716038..5717228	+	1191	WP_004880242.1	methionine adenosyltransferase	-
KHW14_RS25175 (KHW14_25175)	5717315..5717662	-	348	WP_069145198.1	type II toxin-antitoxin system ChpB family toxin	Toxin
KHW14_RS25180 (KHW14_25180)	5717659..5717913	-	255	WP_122409396.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KHW14_RS25185 (KHW14_25185)	5718157..5719839	+	1683	WP_213673986.1	NAD-dependent DNA ligase LigB	-
KHW14_RS25190 (KHW14_25190)	5719894..5720301	+	408	WP_043305551.1	DUF1090 domain-containing protein	-
KHW14_RS25195 (KHW14_25195)	5720637..5721038	-	402	WP_004880251.1	MAPEG family protein	-
KHW14_RS25200 (KHW14_25200)	5721136..5722527	-	1392	WP_043305552.1	heavy metal sensor histidine kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-114)

Antitoxin

• SpoVT-AbrB domain (6-48)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12189.88 Da
• Isoelectric Point: 9.1004

>T202063 WP_069145198.1 NZ_CP074412:c5717662-5717315 [Pseudomonas viridiflava]
VKRPGFNRADIVRINLNPTAGREQQGDFRPALVLTPAAYNVSGLAIVAPITQGGDFARYAGFAVPLSGSGTETQGVVLCN
QIRTVDLQARGAKRIESAPDVVINDALARVQALFE

Nucleotide

• Length: 348 bp

>T202063 NZ_CP099304:c4760125-4759868 [Klebsiella michiganensis]
ATGAATCTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCTAAAGGTGGCCGGATCGCGCACATCCGCGATGCATC
AGGCCGCGTCACCTCTGTTGAATGCTACAGCCGCGAAGGGCTCCTGCTCAGCGACTGTACGCTCGCCGTTTTCAAAAAAC
TCAAAACCAAAAAGCTGATTAAGTCCGTCAACGGACAGCCCTACCGCATCAATACCACCGGGCTGAATAACGTCCGCGCA
CAGCTGGACAATCGCTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9267.69 Da
• Isoelectric Point: 4.6471

>AT202063 WP_122409396.1 NZ_CP074412:c5717913-5717659 [Pseudomonas viridiflava]
MEVKIQQWGNSAAIRLPANVLKQMSLVSGDVLVLDVSSEVMTLKPAKARLHYQLTDLMAQCDLNASEPEELAGWNTMQPT
GREV

Nucleotide

• Length: 255 bp

>AT202063 NZ_CP099304:c4759857-4759306 [Klebsiella michiganensis]
ATGACAAACCACAGCTTCACTTTTCATGTCACCTCTGAAGGCGATACCGATGACATTCTCGACGTCGAAACCCGGGCTTT
CGGCTACAGCAAAGAGGCCAGGCTGGTGGCCGATTTGCTCAACGATGAGAGCGCCTGCCCGACGTTATCGCTGCTGGCCA
GGCATAACGGAGAGGCGGTTGGGCATATTTTATTCACCCGGGCGACGTTCAAAGGGGAGCCGGACTCGCCGATGATGCAC
ATCCTTGCTCCCCTGACGGTGGTGCCGGAATACCAGGGCGCGGGCGTTGGCGGAGAGCTTATCCGGCACGGCATTGAGCA
GTTGAAAGCAATGGGAAGCCAGGCGGTATTTGTTCTGGGCCACGCCGCTTACTACCCCAGGCACGGGTTTGAGCCAGGCG
CGGGAGATAAAGGCTATCCGGCGCCTTATCCGATTCCCGCGGCGCATAAAGCCTGCTGGATGCTGCAGCCGATTTCGTCT
CAGCCGCTGGGACGAACGGGCCAGATACAGTGCGCCCGGGCGTTAATGAAACCCGAGCACTGGCGCGAGTAA