Detailed information of TA system TA202061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 591583..592462
• Replicon: chromosome
• Accession: NZ_CP074412
• Organism: Pseudomonas viridiflava strain U625

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KHW14_RS02625
• Protein ID: WP_004882682.1
• Coordinates: 591583..591936 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KHW14_RS02630
• Protein ID: WP_025993919.1
• Coordinates: 591989..592462 (+)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHW14_RS02600 (KHW14_02600)	588169..588792	-	624	WP_025993915.1	SCP2 sterol-binding domain-containing protein	-
KHW14_RS02605 (KHW14_02605)	588792..589562	-	771	WP_004882686.1	bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE	-
KHW14_RS02610 (KHW14_02610)	589707..589985	+	279	WP_004882685.1	polyhydroxyalkanoic acid system family protein	-
KHW14_RS02615 (KHW14_02615)	590146..590619	+	474	WP_029243151.1	phasin family protein	-
KHW14_RS02620 (KHW14_02620)	590630..591403	+	774	WP_122433123.1	phasin family protein	-
KHW14_RS02625 (KHW14_02625)	591583..591936	+	354	WP_004882682.1	type II toxin-antitoxin system HigB family toxin	Toxin
KHW14_RS02630 (KHW14_02630)	591989..592462	+	474	WP_025993919.1	transcriptional regulator	Antitoxin
KHW14_RS02635 (KHW14_02635)	592639..593253	-	615	WP_004882680.1	TetR/AcrR family transcriptional regulator	-
KHW14_RS02640 (KHW14_02640)	593334..595019	-	1686	WP_029243147.1	class II poly(R)-hydroxyalkanoic acid synthase	-
KHW14_RS02645 (KHW14_02645)	595184..596041	-	858	WP_029243146.1	poly(3-hydroxyalkanoate) depolymerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (73-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13586.75 Da
• Isoelectric Point: 10.0467

>T202061 WP_004882682.1 NZ_CP074412:591583-591936 [Pseudomonas viridiflava]
MRVIAKTTVAKAIEAHSQWKAALSLWLTTFDRATLRFESFEQLRDTWVTTSGWNVDRIPCSKLRQPSLKGPLDVYVFDIK
KNECRIIAWINTRNGTIYIKDILPHAGYDKWCKSDVK

Nucleotide

• Length: 354 bp

>T202061 NZ_CP099304:2249011-2249114 [Klebsiella michiganensis]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17265.63 Da
• Isoelectric Point: 4.9833

>AT202061 WP_025993919.1 NZ_CP074412:591989-592462 [Pseudomonas viridiflava]
MKHDKNGATGLDSFIANISPMLDGLNDQIATMAQLLAACHDPVKSDTDLKGRMALINELYSHADSEDHAAARFAELVAER
VYEYETETVLIPYSSQAEALAFLISDRGVRQKDLSGVATQSAVSEILNNKRKMTVAQIKGFAEFFKVPVEFFMHGVV

Nucleotide

• Length: 474 bp

>AT202061 NZ_CP099304:c2249121-2248953 [Klebsiella michiganensis]
AACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTTACCGTGTCAGGTTTTCCAGTCCGCGACAAAAGTG
GACGAATAA