Detailed information of TA system TA202060

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 5568222..5568741
• Replicon: chromosome
• Accession: NZ_CP074411
• Organism: Pseudomonas syringae strain Susan762

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KHW13_RS23970
• Protein ID: WP_046267076.1
• Coordinates: 5568222..5568512 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S6UWW5
• Locus tag: KHW13_RS23975
• Protein ID: WP_002551444.1
• Coordinates: 5568502..5568741 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHW13_RS23945 (KHW13_23945)	5564164..5565111	-	948	Protein_4724	hypothetical protein	-
KHW13_RS25560	5565300..5565692	-	393	WP_229636537.1	hypothetical protein	-
KHW13_RS23955 (KHW13_23955)	5566013..5567098	+	1086	WP_003342475.1	type I restriction enzyme HsdR N-terminal domain-containing protein	-
KHW13_RS23960 (KHW13_23960)	5567254..5567991	+	738	WP_046267075.1	hypothetical protein	-
KHW13_RS25565	5567898..5568134	-	237	Protein_4728	N-acetyltransferase	-
KHW13_RS23970 (KHW13_23970)	5568222..5568512	-	291	WP_046267076.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KHW13_RS23975 (KHW13_23975)	5568502..5568741	-	240	WP_002551444.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
KHW13_RS23980 (KHW13_23980)	5568903..5569376	-	474	WP_046267077.1	GNAT family N-acetyltransferase	-
KHW13_RS23985 (KHW13_23985)	5569475..5570071	-	597	WP_229699141.1	ATP-binding protein	-
KHW13_RS23990 (KHW13_23990)	5570115..5570684	-	570	WP_046267078.1	GNAT family N-acetyltransferase	-
KHW13_RS23995 (KHW13_23995)	5570809..5571339	+	531	WP_003427268.1	hypothetical protein	-
KHW13_RS24000 (KHW13_24000)	5571377..5572651	-	1275	WP_003420302.1	OprD family porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-81)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-64)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11233.12 Da
• Isoelectric Point: 9.9227

>T202060 WP_046267076.1 NZ_CP074411:c5568512-5568222 [Pseudomonas syringae]
MTYNLEFDARALKEWHKLGDTVRQQLKKKLATILVAPRVEANRLHALPDCYKIKLRSSGYRLVYQVIDQEVVVFVVAVDK
REREEVYCKAADRLGR

Nucleotide

• Length: 291 bp

>T202060 NZ_CP099304:2080865-2081197 [Klebsiella michiganensis]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTGGATCCGATTGCAGGCCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGTTACCCGTTGTTGTTCCCATCACCAGCGGCGGAAATTTTGCTCGCA
CGGCAGGTTTCACCGTTTCACTGGAAGGGGCAGGGATAAAAACGTTGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGACTGCCCGGAACGGCAAGCGGCTGGAACGCATACCAGACGCTGTGGTAAATGAAGTGCTTGCCAGGCTGGATGC
AATTTTGAGTTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8955.34 Da
• Isoelectric Point: 4.6017

>AT202060 WP_002551444.1 NZ_CP074411:c5568741-5568502 [Pseudomonas syringae]
MASINVRIDDDLKARAYLELEKLGVTPSELLRQTLQYVAERGQLPFKTVLMTEEDEALLATVRERLAAPQRVKVSLDDL

Nucleotide

• Length: 240 bp

>AT202060 NZ_CP099304:2080607-2080864 [Klebsiella michiganensis]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTCAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTCGGCATGACCATTAATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCTCATTATTCTC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCTCCTGCGGTG
GGCAAGGAGATCCTGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0K8M1G9