Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 913477..914111 Replicon chromosome
Accession NZ_CP074411
Organism Pseudomonas syringae strain Susan762

Toxin (Protein)


Gene name vapC Uniprot ID A0A193SM35
Locus tag KHW13_RS04055 Protein ID WP_003345997.1
Coordinates 913477..913881 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A3M4XXE1
Locus tag KHW13_RS04060 Protein ID WP_003345999.1
Coordinates 913881..914111 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHW13_RS04025 (KHW13_04025) 908498..908947 - 450 WP_003345986.1 DUF2384 domain-containing protein -
KHW13_RS04030 (KHW13_04030) 909213..910217 + 1005 WP_096113446.1 response regulator -
KHW13_RS04035 (KHW13_04035) 910221..910703 + 483 WP_003345989.1 PAS domain-containing protein -
KHW13_RS04040 (KHW13_04040) 910800..912056 + 1257 WP_024663315.1 3-phosphoshikimate 1-carboxyvinyltransferase -
KHW13_RS04045 (KHW13_04045) 912079..912741 - 663 WP_004402535.1 ChrR family anti-sigma-E factor -
KHW13_RS04050 (KHW13_04050) 912741..913253 - 513 WP_003345995.1 sigma-70 family RNA polymerase sigma factor -
KHW13_RS04055 (KHW13_04055) 913477..913881 - 405 WP_003345997.1 tRNA(fMet)-specific endonuclease VapC Toxin
KHW13_RS04060 (KHW13_04060) 913881..914111 - 231 WP_003345999.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KHW13_RS04065 (KHW13_04065) 914228..915109 - 882 WP_046266959.1 aldose 1-epimerase -
KHW13_RS04070 (KHW13_04070) 915084..916055 - 972 WP_096113445.1 DMT family transporter -
KHW13_RS04075 (KHW13_04075) 916140..917420 - 1281 WP_003421655.1 TRAP transporter large permease -
KHW13_RS04080 (KHW13_04080) 917421..917948 - 528 WP_004402549.1 TRAP transporter small permease -
KHW13_RS04085 (KHW13_04085) 918012..919037 - 1026 WP_096113444.1 TRAP transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-125)

Antitoxin

SpoVT-AbrB domain

(7-34)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14946.21 Da        Isoelectric Point: 6.8620

>T202057 WP_003345997.1 NZ_CP074411:c913881-913477 [Pseudomonas syringae]
MLKYMLDTNICIFTIKNKPTSVREAFNLHHGQLCISAITLMELVYGAEKSLSPERNLAVVEGFTARLEVLPYDSDAAAHT
GMIRAELARSGTPIGPYDQMIAGHARSLGLVVITNNQREFRRVEGLRVEDWVSQ

Download         Length: 405 bp

>T202057 NZ_CP099303:c4623183-4622809 [Citrobacter freundii]
ATGGTCAAAGGATCCGCGCTTTTTGATACCAACATCCTCATCGATCTGTTTAGTGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAATGCAATCAGCCTCATTACATGGATGGAGGTGATGGTGGGTGCAAAAAAATACCATCAGG
AAGCCCGTACTCGTGTTGCGCTAGGTGCTTTTAACGTGATCGGCGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGACAGGAATATGGGATGAAATTGCCGGATGCCATTATTTTGGCAACAGCACAGATTCATCGTCTTACGCTGGTTACGCG
AAATACTAAGGATTTTGCAGGCCTATCCGGTGTGGTCACACCGTATACATTATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8580.59 Da        Isoelectric Point: 4.5557

>AT202057 WP_003345999.1 NZ_CP074411:c914111-913881 [Pseudomonas syringae]
MEQSTVFKSNRSQAVRLPKAVALPDDVKRVDVVAVGRTRIISPAGEMWNSWFDGESVSDDFMAEREQPAEQQRESL

Download         Length: 231 bp

>AT202057 NZ_CP099303:c4623425-4623183 [Citrobacter freundii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAAGTGATTAAACGTCTTGATGATCTCAAGGT
GCAGCGCAACCTGCCGCGAGCAGAACTTTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAGGATCAGGCTGAAACCA
CAATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTCCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A193SM35

Antitoxin

Source ID Structure
AlphaFold DB A0A3M4XXE1

References