Detailed information of TA system TA202056

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 1977959..1978472
• Replicon: chromosome
• Accession: NZ_CP074410
• Organism: Pseudomonas syringae strain U643

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KHW15_RS08860
• Protein ID: WP_103363385.1
• Coordinates: 1977959..1978243 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A3M4E5F0
• Locus tag: KHW15_RS08865
• Protein ID: WP_024648170.1
• Coordinates: 1978233..1978472 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHW15_RS08845 (KHW15_08845)	1974101..1976029	+	1929	WP_016567635.1	methyl-accepting chemotaxis protein	-
KHW15_RS08850 (KHW15_08850)	1976063..1976995	-	933	WP_032660393.1	DMT family transporter	-
KHW15_RS08855 (KHW15_08855)	1977070..1977924	+	855	WP_003423353.1	LysR family transcriptional regulator	-
KHW15_RS08860 (KHW15_08860)	1977959..1978243	-	285	WP_103363385.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KHW15_RS08865 (KHW15_08865)	1978233..1978472	-	240	WP_024648170.1	CopG family ribbon-helix-helix protein	Antitoxin
KHW15_RS08870 (KHW15_08870)	1978632..1979648	-	1017	WP_213569413.1	1-aminocyclopropane-1-carboxylate deaminase	-
KHW15_RS08875 (KHW15_08875)	1979835..1980341	+	507	WP_003315203.1	Lrp/AsnC family transcriptional regulator	-
KHW15_RS08880 (KHW15_08880)	1980489..1981397	+	909	WP_032660395.1	DUF808 domain-containing protein	-
KHW15_RS08885 (KHW15_08885)	1981574..1982359	-	786	WP_003396313.1	outer membrane protein OmpK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-89)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-41)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10659.37 Da
• Isoelectric Point: 10.4249

>T202056 WP_103363385.1 NZ_CP074410:c1978243-1977959 [Pseudomonas syringae]
MQVEWLKTALKNLDEEAAYIALDNPAAAAAFVKAIQSNVTQLASFPAMGREGRIAGTREWPLPDLPYLIPYRIRSGRLQV
LRIFHTRRQSPPVW

Nucleotide

• Length: 285 bp

>T202056 NZ_CP099303:c4471947-4471588 [Citrobacter freundii]
ATGACGAAACCACTTTACTGGGTTGGGCAGGCCAGAAAAGACTTGCTTGCAATGCCAGAACACGTTCGGGATACGTTTGG
GTTTGCTCTTTGGCTTGCGCAGCAGGGTAAACAGCATTCACAGACTAAACCATTAAAAGGATTTGGTGGAGCAGGGGTTC
TGGAGGTCGTTGAGGATTACCACGGTAGCGCCTGGCGGGCGGTTTACACCATCCAGTTGAAAAATGCGGTTTATGTTCTC
CATGTTTTTCAGAAGAAATCCGTTTCCGGAAAGGCAACCCCGAAACCTGAAATTGACCTGATTTATCAGCGGCTCAAGGC
TGCACAGCGCCATGCGCAGGAGAGTGGATATGTCACATGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8715.80 Da
• Isoelectric Point: 4.4354

>AT202056 WP_024648170.1 NZ_CP074410:c1978472-1978233 [Pseudomonas syringae]
MSTMSLRLPDEMADTLAHLAKATGRSKSFLALDALREYLTREAWQIAEIQRAIEEADAGEFASQEDVKAVMDKWSGNAG

Nucleotide

• Length: 240 bp

>AT202056 NZ_CP099303:c4471598-4471281 [Citrobacter freundii]
ATGTCACATGAAGTGGAAGCTAGTAGCGGTAATGTTTACGCCGATTTAGGCGCGTATGACGCAGAACAGATGCAGTTGAA
AGCGCAACTGGCAATGGTGATTGGTGAGATTTTGCAACGCAAAGGGTTAACGCAGCAGCAGGCTGCGCAGCTACTGGGAA
TGACGCAGCCTAAACTTTCCAACCTGCTACGTGGTCAGTTTCGTGGTATCAGTGAAGCAAAGATGCTCGAATGCCTGGTG
AGACTGGGCCGTGATGTAGAGATTGTTGTCGGGGAAGAGGGCAATATGTCAGGAAAGCTGAAAGTGAAATTTGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3M4E5F0